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Selection of ESBL-Producing E. coli in a Mouse Intestinal Colonization Model

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  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

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  2. Analysis of Mass Cytometry Data

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  3. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

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  4. Full-Length Open Reading Frame Amplification of Hepatitis C Virus

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  5. In Vitro Neutralization Assay Using Cultured Hepatitis C Virus

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  1. Exposure of consumers to substandard antibiotics from selected authorised and unauthorised medicine sales outlets in Ghana

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  2. Oral amoxicillin and amoxicillin–clavulanic acid: properties, indications, and usage

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  3. A clear conscience is the sure sign of a bad memory: Vancomycin-resistant enterococci and rectal thermometers

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  4. Effects of Antibiotics on the Intestinal Microbiota of Mice

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  5. Antibiotic-prescribing and antibiotic-resistance patterns among elderly citizens residing in two Nordic regions

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Asymptomatic human carriage of antimicrobially drug-resistant pathogens prior to infection is increasing worldwide. Further investigation into the role of this fecal reservoir is important for combatting the increasing antimicrobial resistance problems. Additionally, the damage on the intestinal microflora due to antimicrobial treatment is still not fully understood. Animal models are powerful tools to investigate bacterial colonization subsequent to antibiotic treatment. In this chapter we present a mouse-intestinal colonization model designed to investigate how antibiotics select for an ESBL-producing E. coli isolate. The model can be used to study how antibiotics with varying effect on the intestinal flora promote the establishment of the multidrug-resistant E. coli. Colonization is successfully investigated by sampling and culturing stool during the days following administration of antibiotics. Following culturing, a precise identification of the bacterial strain found in mice feces is applied to ensure that the isolate found is in fact identical to the strain used for inoculation. For this purpose random amplified of polymorphic DNA (RAPD) PCR specifically developed for E. coli is applied. This method allows us to distinguish E. coli with more than 99.95% genome similarity using a duplex PCR method.

Original languageEnglish
JournalMethods in molecular biology
Volume1736
Pages (from-to)105-115
Number of pages11
ISSN1064-3745
DOIs
Publication statusPublished - 2018

ID: 55383931