Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Selecting Infants With Cryptorchidism and High Risk of Infertility for Optional Adjuvant Hormonal Therapy and Cryopreservation of Germ Cells: Experience From a Pilot Study

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Editorial: Novel Biomarkers for Type 2 Diabetes

    Research output: Contribution to journalEditorialResearchpeer-review

  2. Editorial: The Role of Genetic and Lifestyle Factors in Metabolic Diseases

    Research output: Contribution to journalEditorialResearchpeer-review

  3. Liraglutide-Induced Weight Loss May be Affected by Autonomic Regulation in Type 1 Diabetes

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. In-utero Exposure to Maternal Stressful Life Events and Risk of Cryptorchidism: The Raine Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Laparoscopy to Assist Surgical Decisions Related to Necrotizing Enterocolitis in Preterm Neonates

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Gene Expression in Granulosa Cells From Small Antral Follicles From Women With or Without Polycystic Ovaries

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Quantitative Differences in TGF-β Family Members Measured in Small Antral Follicle Fluids From Women With or Without PCO

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Gonocyte transformation in congenital undescended testes: what is the role of inhibin-B in cell death?

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Introduction: Orchiopexy for congenital cryptorchid testes is recommended between ½ and 1 year of age to preserve testicular germ cell maturation. Early operation is not enough to preserve fertility in 22 and 36% of cases. Aim of this study was to set up a protocol for optional adjuvant hormonal therapy after orchiopexy and thereafter cryopreservation of testicular biopsies from infants with bilateral cryptorchidism and high infertility risk.

Materials and methods: We included 17 boys with bilateral cryptorchidism, normal FSH, and impaired germ cell number per tubular transverse section (G/T) in testicular biopsies at orchiopexy, 7 months to 3½ years old. Postoperatively, optional adjuvant LHRH (kryptocur®) 0.2 mg/0.1 mL 2× every second day in 16 weeks were offered. Ten boys were applicable for age matching according to parent's choice of treatment regime and G/T. Five of them had kryptocur®, and five were controls. Repeat bilateral testicular biopsy evaluation and cryopreservation were offered to all boys 12 months after primary orchiopexy. For cryopreservation, tissue pieces were incubated with a cryoprotectant with a slow program freezing.

Results: Two out of five kryptorcur®-treated boys normalized both the average G/T and the number of adult dark spermatogonia (Ad-S). Another kryptocur®-treated boy with initial low G/T and no Ad-S increased the G/T and achieved normal number of Ad-S at time of cryopreservation. In the control group, two patients reached only normal lower range regarding the G/T and the number of Ad-S. None of boys with less than average 0.2 G/T improved significantly, whether they were kryptocur®-treated or not.

Conclusion: Based on literature and the present results, we recommend adjuvant LHRH treatment to boys with cryptorchidism and insufficient genuine gonadotropin stimulation at time of surgery, as these patients have high infertility risk. Cryopreservation should be an option in case of treatment failure of adjuvant LHRH. However, to avoid repeat surgery with biopsy, some parents may choose biopsy for cryopreservation at time of the initial bilateral orchiopexy, well informed that the procedure may only be truly indicated in 22 and 36% of the cases.

Original languageEnglish
JournalFrontiers in Endocrinology
Volume9
Pages (from-to)299
ISSN1664-2392
DOIs
Publication statusPublished - 1 Jun 2018

ID: 54652525