Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Automatic airway segmentation from computed tomography using robust and efficient 3-D convolutional neural networks

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Detection of biological signals from a live mammalian muscle using an early stage diamond quantum sensor

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Infants with congenital heart defects have reduced brain volumes

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Presence of SARS-CoV-2 in semen and the impact of COVID-19 on the male reproductive system

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Point of care testing for infectious disease: ownership and quality

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. A comparison of predictors for mortality and bacteraemia in patients suspected of infection

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Maiken W Rosenstierne
  • Shreya Joshi
  • E Thomas Danielsen
  • Helen Webb
  • Dac Mui Luong
  • Julie Bjerring
  • Julie Hindkær
  • Lærke Jørgensen
  • Julie Blauenfeldt
  • Ask Bojesen
  • Flemming Holck
  • Johnny Weber Lau
  • Lars Bangsgaard
  • Jakob Broberg Lind
  • Mette Bjergaard Dragheim
  • Mikkel Rohde Jacobsen
  • Robert Elkjær
  • Steven Clauwaert
  • Kristina Christensen
  • Charlotta Polacek
  • Anders Fomsgaard
  • Tuomas Ojalehto
  • Antti Tullila
  • Mirko Brummer
  • Claus Juel Jensen
  • Frederikke Holm Jensen
  • Uffe Vest Schneider
  • Jan Gorm Lisby
  • Rikke Lind Jørgensen
  • Thomas Warthoe
  • Ebbe Finding
  • Peter Warthoe
View graph of relations

Rapid nucleic-acid based tests that can be performed by non-professionals outside laboratory settings could help the containment of the pandemic SARS-CoV-2 virus and may potentially prevent further widespread lockdowns. Here, we present a novel compact portable detection instrument (the Egoo Health System) for extraction-free detection of SARS-CoV-2 using isothermal reverse transcription strand invasion based amplification (RT-SIBA). The SARS-CoV-2 RT-SIBA assay can be performed directly on crude oropharyngeal swabs without nucleic acid extraction with a reaction time of 30 min. The Egoo Health system uses a capsule system, which is automatically sealed tight in the Egoo instrument after applying the sample, resulting in a closed system optimal for molecular isothermal amplification. The performance of the Egoo Health System is comparable to the PCR instrument with an analytical sensitivity of 25 viral RNA copies per SARS-CoV-2 RT-SIBA reaction and a clinical sensitivity and specificity between 87.0-98.4% and 96.6-98.2% respectively.

Original languageEnglish
Article number22214
JournalScientific Reports
Volume11
Issue number1
Pages (from-to)1-13
Number of pages13
ISSN2045-2322
DOIs
Publication statusPublished - 15 Nov 2021

Bibliographical note

© 2021. The Author(s).

    Research areas

  • COVID-19/diagnosis, Cell Phone, Equipment Design, Humans, Mobile Applications, Molecular Diagnostic Techniques/instrumentation, Oropharynx/virology, Pandemics/prevention & control, Point-of-Care Testing, Polymorphism, Single Nucleotide, RNA, Viral/genetics, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction/instrumentation, SARS-CoV-2/genetics, Sensitivity and Specificity

ID: 69062673