RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Mohsen Mohammadniaei; Ming Zhang; Jon Ashley; Ulf Bech Christensen; Lennart Jan Friis-Hansen; Rasmus Gregersen; Jan Gorm Lisby; Thomas Lars Benfield; Finn Erland Nielsen; Jens Henning Rasmussen; Ellen Bøtker Pedersen; Anne Christine Rye Olinger; Lærke Tørring Kolding; Maryam Naseri; Tao Zheng; Wentao Wang; Jan Gorodkin; Yi Sun

doi:10.1038/s41467-021-25387-9

RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Mohsen Mohammadniaei, Ming Zhang, Jon Ashley, Ulf Bech Christensen, Lennart Jan Friis-Hansen, Rasmus Gregersen, Jan Gorm Lisby, Thomas Lars Benfield, Finn Erland Nielsen, Jens Henning Rasmussen, Ellen Bøtker Pedersen, Anne Christine Rye Olinger, Lærke Tørring Kolding, Maryam Naseri, Tao Zheng, Wentao Wang, Jan Gorodkin, Yi Sun

58 Citations (Scopus)

Abstract

The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL-1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

Original language	English
Article number	5089
Journal	Nature Communications
Volume	12
Issue number	1
Pages (from-to)	1-12
Number of pages	12
DOIs	https://doi.org/10.1038/s41467-021-25387-9 https://doi.org/10.1038/s41467-025-59976-9
Publication status	Published - 24 Aug 2021

Keywords

COVID-19 Nucleic Acid Testing/methods
COVID-19 Testing
COVID-19/diagnosis
Humans
Nucleic Acid Amplification Techniques/methods
RNA, Viral/genetics
Recombination, Genetic
SARS-CoV-2/genetics

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Mohammadniaei, M., Zhang, M., Ashley, J., Christensen, U. B., Friis-Hansen, L. J., Gregersen, R., Lisby, J. G., Benfield, T. L., Nielsen, F. E., Henning Rasmussen, J., Pedersen, E. B., Olinger, A. C. R., Kolding, L. T., Naseri, M., Zheng, T., Wang, W., Gorodkin, J., & Sun, Y. (2021). RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications, 12(1), 1-12. Article 5089. https://doi.org/10.1038/s41467-021-25387-9, https://doi.org/10.1038/s41467-025-59976-9

Mohammadniaei, M, Zhang, M, Ashley, J, Christensen, UB, Friis-Hansen, LJ , Gregersen, R , Lisby, JG , Benfield, TL, Nielsen, FE, Henning Rasmussen, J , Pedersen, EB, Olinger, ACR, Kolding, LT, Naseri, M, Zheng, T, Wang, W, Gorodkin, J & Sun, Y 2021, 'RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA', Nature Communications, vol. 12, no. 1, 5089, pp. 1-12. https://doi.org/10.1038/s41467-021-25387-9, https://doi.org/10.1038/s41467-025-59976-9

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AU - Henning Rasmussen, Jens

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AU - Kolding, Lærke Tørring

AU - Naseri, Maryam

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AU - Gorodkin, Jan

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RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

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