Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The Optical Fractionator Technique to Estimate Cell Numbers in a Rat Model of Electroconvulsive Therapy

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Exploration of physiological and pathophysiological implications of miRNA-143 and miRNA-145 in cerebral arteries

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Altered surface expression of P2Y11 receptor with narcolepsy-associated mutations

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Does inflammation have a role in migraine?

    Research output: Contribution to journalReviewResearchpeer-review

  4. C-fibers may modulate adjacent Aδ-fibers through axon-axon CGRP signaling at nodes of Ranvier in the trigeminal system

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Distribution of CGRP and CGRP receptor components in the rat brain

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Retinal pericytes play an important role in many diseases of the eye. Immunohistochemical staining techniques of retinal vessels and microvascular pericytes are central to ophthalmological research. It is vital to choose an appropriate method of visualizing the microvascular pericytes. We describe retinal microvascular pericyte immunohistochemical staining in cryo-sections, whole-mounts, and hypotonic isolated vasculature using antibodies for platelet-derived growth factor receptor β (PDGFRβ) and nerve/glial antigen 2 (NG2). This allows us to highlight advantages and shortcomings of each of the three tissue preparations for the visualization of the retinal microvascular pericytes. Cryo-sections provide transsectional visualization of all retinal layers but contain only a few occasional transverse cuts of the microvasculature. Whole-mount provides an overview of the entire retinal vasculature, but visualization of the microvasculature can be troublesome. Hypotonic isolation provides a method to visualize the entire retinal vasculature by the removal of neuronal cells, but this makes the tissue very fragile.

Original languageEnglish
JournalJournal of visualized experiments : JoVE
Issue number140
Pages (from-to)e57733
Number of pages6
ISSN1940-087X
DOIs
Publication statusPublished - 2018

ID: 55617472