Reporter gene assay for the quantification of the activity and neutralizing antibody response to TNFα antagonists

Christophe Lallemand, Nadia Kavrochorianou, Casper Steenholdt, Klaus Bendtzen, Mark A Ainsworth, Jean-Francois Meritet, Brigitte Blanchard, Pierre Lebon, Peter Taylor, Peter Charles, Saba Alzabin, Michael G Tovey

84 Citations (Scopus)


A cell-based assay has been developed for the quantification of the activity of TNFα antagonists based on human erythroleukemic K562 cells transfected with a NFκB regulated firefly luciferase reporter-gene construct. Both drug activity and anti-drug neutralizing antibodies can be quantified with a high degree of precision within 2h, and without interference from cytokines and other factors known to activate NFκB. The assay cells also contain the Renilla luciferase reporter gene under the control of a constitutive promoter that allows TNFα-induced firefly luciferase activity to be normalized relative to Renilla luciferase expression. Thus, results are independent of cell number or differences in cell viability, resulting in intra and inter assay coefficients of variation of 10% or less. Normalization of results relative to the expression of an internal standard also provides a means for correcting for serum matrix effects and allows residual drug levels or anti-drug neutralizing antibodies to be quantified even in serum samples with a relatively high degree of cytotoxicity.
Original languageEnglish
JournalJournal of Immunological Methods
Issue number1-2
Pages (from-to)229-39
Number of pages11
Publication statusPublished - 2011


  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Humanized
  • Antibodies, Neutralizing
  • Arthritis, Rheumatoid
  • Crohn Disease
  • Culture Media
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation, Enzymologic
  • Humans
  • K562 Cells
  • Luciferases
  • Luciferases, Firefly
  • Luciferases, Renilla
  • NF-kappa B
  • Recombinant Fusion Proteins
  • Regulatory Sequences, Nucleic Acid
  • Serum
  • Tumor Necrosis Factor-alpha


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