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Regulation of urokinase receptors in monocytelike U937 cells by phorbol ester phorbol myristate acetate

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  1. Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability

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  2. Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins

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  3. Comparison of two different culture conditions for derivation of early hiPSC

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  4. M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway

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  5. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery

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  1. Tumor cell MT1-MMP is dispensable for osteosarcoma tumor growth, bone degradation and lung metastasis

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  2. The collagen receptor uPARAP/Endo180 regulates collectins through unique structural elements in its FNII domain

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  3. Cellular uptake of collagens and implications for immune cell regulation in disease

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  4. TAFI deficiency causes maladaptive vascular remodeling after hemophilic joint bleeding

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  5. CCL2/MCP-1 signaling drives extracellular matrix turnover by diverse macrophage subsets

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A specific surface receptor for urokinase plasminogen activator (uPA) recognizes the amino-terminal growth factor-like sequence of uPA, a region independent from and not required for the catalytic activity of this enzyme. The properties of the uPA receptor (uPAR) and the localization and distribution of uPA in tumor cells and tissues suggest that the uPA/uPAR interaction may be important in regulating extracellular proteolysis-dependent processes (e.g., invasion, tissue destruction). Phorbol myristate acetate (PMA), an inducer of U937 cell differentiation to macrophage-like cells, elicits a time- and concentration-dependent increase in the number of uPAR molecules as shown by binding, cross-linking, and immunoprecipitation studies. The effect of PMA is blocked by cycloheximide. Overall, the data indicate that PMA increases the synthesis of uPA. PMA treatment also causes a decrease in the affinity of the uPAR for uPA, thus uncovering another way of regulating the interaction between uPA and uPAR. In addition, the PMA treatment causes a modification of migration of the cross-linked receptor in mono- and bidimensional gel electrophoresis.

Original languageEnglish
JournalJournal of Cell Biology
Volume108
Issue number2
Pages (from-to)693-702
Number of pages10
ISSN0021-9525
Publication statusPublished - Feb 1989

    Research areas

  • Cell Count, Cell Line, Cross-Linking Reagents, Electrophoresis, Polyacrylamide Gel, Enzyme Precursors, Humans, Immunosorbent Techniques, Molecular Weight, Monocytes, Peptide Fragments, Plasminogen Activators, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Tetradecanoylphorbol Acetate, Urokinase-Type Plasminogen Activator

ID: 46434287