Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Regulation of axon guidance by compartmentalized nonsense-mediated mRNA decay

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Low-Frequency and Rare-Coding Variation Contributes to Multiple Sclerosis Risk

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Genomic Dissection of Bipolar Disorder and Schizophrenia, Including 28 Subphenotypes

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Drug-Driven Phenotypic Convergence Supports Rational Treatment Strategies of Chronic Infections

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. CGRP Antibodies as Prophylaxis in Migraine

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Genetic and Functional Drivers of Diffuse Large B Cell Lymphoma

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. The splicing factor RBM25 controls MYC activity in acute myeloid leukemia

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Leukemogenic nucleophosmin mutation disrupts the transcription factor hub regulating granulo-monocytic fates

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Human adult HSCs can be discriminated from lineage-committed HPCs by the expression of endomucin

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
Growth cones enable axons to navigate toward their targets by responding to extracellular signaling molecules. Growth-cone responses are mediated in part by the local translation of axonal messenger RNAs (mRNAs). However, the mechanisms that regulate local translation are poorly understood. Here we show that Robo3.2, a receptor for the Slit family of guidance cues, is synthesized locally within axons of commissural neurons. Robo3.2 translation is induced by floor-plate-derived signals as axons cross the spinal cord midline. Robo3.2 is also a predicted target of the nonsense-mediated mRNA decay (NMD) pathway. We find that NMD regulates Robo3.2 synthesis by inducing the degradation of Robo3.2 transcripts in axons that encounter the floor plate. Commissural neurons deficient in NMD proteins exhibit aberrant axonal trajectories after crossing the midline, consistent with misregulation of Robo3.2 expression. These data show that local translation is regulated by mRNA stability and that NMD acts locally to influence axonal pathfinding.
Original languageEnglish
JournalCell
Volume153
Issue number6
Pages (from-to)1252-65
Number of pages14
ISSN0092-8674
DOIs
Publication statusPublished - 6 Jun 2013

    Research areas

  • Animals, Axons, Embryo, Mammalian, Growth Cones, Membrane Proteins, Mice, Nerve Tissue Proteins, Neurons, Nonsense Mediated mRNA Decay, Protein Biosynthesis, RNA Isoforms, RNA Stability, Spinal Cord

ID: 40038196