Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Reduced Sublingual Endothelial Glycocalyx in Type 1 Diabetic Patients with Diabetic Nephropathy

Research output: Contribution to journalConference abstract in journalResearchpeer-review

  1. ASN Kidney Week 2017

    Activity: Participating in or organising an eventOrganisation of and participation in conference

  1. Essential Branched-Chain Amino Acids and Ribonic Acid Are Associated with Cardiorenal Events in Type 1 Diabetes

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

  2. Genome-Wide Association Study of Diabetic Kidney Disease Highlights Biology Involved in Glomerular Basement Membrane Collagen

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Assessment of the Sublingual Microcirculation with the GlycoCheck System: Reproducibility and Examination Conditions

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

  4. Hypoxia in individuals with type 1 diabetes and macroalbuminuria is associated with autonomic dysfunction

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

View graph of relations
Background Glycocalyx is a glycoprotein layer that lines and protects the capillary endothelium. Damage to the glycocalyx may be an early stage in development of microvascular complications in diabetes. Insight into the function and thickness of glycocalyx in vivo, has been limited by the lack of easy and non-invasive quantification tools. With capillaroscopy it is possible to visualize the sublingual capillaries by sidestream dark field imaging and estimates the dimensions of the glycocalyx by measuring the perfused boundary region (PBR). We evaluated the glycocalyx thickness non-invasively in type 1 diabetic patients with different levels of historical and current albuminuria. Methods Cross-sectional study including 77 type 1 diabetic patients stratified by history of normoalbuminuria (<30 mg/g;n=26), microalbuminuria (30-299 mg/g;n=27) and macroalbuminuria (>300 mg/g;n=24). Glycocalyx thickness was assessed by 5 measurements with the GlucoCheck device (GlucoCheck BV, The Netherlands), a non-invasive hand-held microscope generating video recordings of the sublingual capillaries. Endothelial glycocalyx thickness was estimated from the PBR in capillaries with a diameter range of 5-25 μm. Higher PBR indicates smaller glycocalyx width. Urinary albumin-to-creatinine ratio (UACR) was measured in 3 morning samples. Results In normo-, micro-, and macroalbuminuric patients PBR was (mean±SD) 2.30±0.22 μm, 2.32±0.25 μm, and 2.49±0.35 μm, respectively. Differences between normo- and macroalbuminuric patients (p=0.020) and micro- and macroalbuminuric patients (p=0.042) were significant, but the difference between normo- and microalbuminuric patients was not (p=0.74). After adjustment for age, sex, HbA1c, diabetes duration and systolic blood pressure, differences between normo- and macroalbuminuric patients (p=0.018) and micro- and macroalbuminuric patients (p=0.004) remained significant. In pooled (n=77) multivariate linear regression, higher level of current UACR was associated with a higher PBR (p=0.0007). Conclusion In type 1 diabetic patients with a history of macroalbuminuria, measurements with the non-invasive GlucoCheck device revealed significantly higher PBR, suggesting an impaired glycocalyx, compared to patients with normo- or microalbuminuria. Moreover, higher current level of albuminuria was associated with higher PBR
Original languageEnglish
Article numberFR-PO666
JournalJournal of the American Society of Nephrology
Volume28
Pages (from-to)575
Number of pages1
ISSN1046-6673
Publication statusPublished - 3 Nov 2017
EventASN Kidney Week 2017 - Ernest N. Morial Convention Center, New Orleans, United States
Duration: 31 Oct 20175 Nov 2017
https://www.asn-online.org/kidneyweek/

Conference

ConferenceASN Kidney Week 2017
LocationErnest N. Morial Convention Center
CountryUnited States
CityNew Orleans
Period31/10/201705/11/2017
Internet address

Event

ASN Kidney Week 2017

31/10/201705/11/2017

New Orleans, United States

Event: Conference

ID: 58494779