Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Anja Lindis Dahl; Martin Barfred Friis; Hanne Wiese Hallberg; Gitte Qvist Kristiansen; Barbara Juliane Holzknecht

doi:10.1016/j.diagmicrobio.2024.116233

Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Anja Lindis Dahl^*, Martin Barfred Friis, Hanne Wiese Hallberg, Gitte Qvist Kristiansen, Barbara Juliane Holzknecht

^*Corresponding author for this work

1 Citation (Scopus)

Abstract

Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

Original language	English
Article number	116233
Journal	Diagnostic Microbiology and Infectious Disease
Volume	109
Issue number	2
Pages (from-to)	116233
ISSN	0732-8893
DOIs	https://doi.org/10.1016/j.diagmicrobio.2024.116233
Publication status	Published - Jun 2024

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10.1016/j.diagmicrobio.2024.116233Licence: CC BY

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title = "Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth",

abstract = "Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 \% inconclusive), we found an excellent sensitivity of 98,3 \% and specificity of 99,7 \% for the remaining 497 samples.",

author = "Dahl, \{Anja Lindis\} and Friis, \{Martin Barfred\} and Hallberg, \{Hanne Wiese\} and Kristiansen, \{Gitte Qvist\} and Holzknecht, \{Barbara Juliane\}",

year = "2024",

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AU - Dahl, Anja Lindis

AU - Friis, Martin Barfred

AU - Hallberg, Hanne Wiese

AU - Kristiansen, Gitte Qvist

AU - Holzknecht, Barbara Juliane

PY - 2024/6

Y1 - 2024/6

N2 - Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

AB - Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vanB vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.

UR - https://www.scopus.com/pages/publications/85189180682

U2 - 10.1016/j.diagmicrobio.2024.116233

DO - 10.1016/j.diagmicrobio.2024.116233

M3 - Journal article

C2 - 38547798

SN - 0732-8893

VL - 109

SP - 116233

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

M1 - 116233

ER -

Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth

Abstract

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