Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Quorum sensing regulation in Aeromonas hydrophila

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Garde, C, Bjarnsholt, T, Givskov, M, Jakobsen, TH, Hentzer, M, Claussen, A, Sneppen, K, Ferkinghoff-Borg, J & Sams, T 2010, 'Quorum sensing regulation in Aeromonas hydrophila' Journal of Molecular Biology, vol. 396, no. 4, pp. 849-57. https://doi.org/10.1016/j.jmb.2010.01.002

APA

Garde, C., Bjarnsholt, T., Givskov, M., Jakobsen, T. H., Hentzer, M., Claussen, A., ... Sams, T. (2010). Quorum sensing regulation in Aeromonas hydrophila. Journal of Molecular Biology, 396(4), 849-57. https://doi.org/10.1016/j.jmb.2010.01.002

CBE

Garde C, Bjarnsholt T, Givskov M, Jakobsen TH, Hentzer M, Claussen A, Sneppen K, Ferkinghoff-Borg J, Sams T. 2010. Quorum sensing regulation in Aeromonas hydrophila. Journal of Molecular Biology. 396(4):849-57. https://doi.org/10.1016/j.jmb.2010.01.002

MLA

Vancouver

Garde C, Bjarnsholt T, Givskov M, Jakobsen TH, Hentzer M, Claussen A et al. Quorum sensing regulation in Aeromonas hydrophila. Journal of Molecular Biology. 2010 Mar 5;396(4):849-57. https://doi.org/10.1016/j.jmb.2010.01.002

Author

Garde, Christian ; Bjarnsholt, Thomas ; Givskov, Michael ; Jakobsen, Tim Holm ; Hentzer, Morten ; Claussen, Anetta ; Sneppen, Kim ; Ferkinghoff-Borg, Jesper ; Sams, Thomas. / Quorum sensing regulation in Aeromonas hydrophila. In: Journal of Molecular Biology. 2010 ; Vol. 396, No. 4. pp. 849-57.

Bibtex

@article{7fb2ee97ad524594bcd64ea1f29c98bb,
title = "Quorum sensing regulation in Aeromonas hydrophila",
abstract = "We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.",
author = "Christian Garde and Thomas Bjarnsholt and Michael Givskov and Jakobsen, {Tim Holm} and Morten Hentzer and Anetta Claussen and Kim Sneppen and Jesper Ferkinghoff-Borg and Thomas Sams",
note = "(c) 2009 Elsevier Ltd. All rights reserved.",
year = "2010",
month = "3",
day = "5",
doi = "10.1016/j.jmb.2010.01.002",
language = "English",
volume = "396",
pages = "849--57",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press",
number = "4",

}

RIS

TY - JOUR

T1 - Quorum sensing regulation in Aeromonas hydrophila

AU - Garde, Christian

AU - Bjarnsholt, Thomas

AU - Givskov, Michael

AU - Jakobsen, Tim Holm

AU - Hentzer, Morten

AU - Claussen, Anetta

AU - Sneppen, Kim

AU - Ferkinghoff-Borg, Jesper

AU - Sams, Thomas

N1 - (c) 2009 Elsevier Ltd. All rights reserved.

PY - 2010/3/5

Y1 - 2010/3/5

N2 - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

AB - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

U2 - 10.1016/j.jmb.2010.01.002

DO - 10.1016/j.jmb.2010.01.002

M3 - Journal article

VL - 396

SP - 849

EP - 857

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 4

ER -

ID: 31052970