Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Quorum sensing regulation in Aeromonas hydrophila

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Lactate-Mediated Protection of Retinal Ganglion Cells

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The Limitations of In Vitro Experimentation in Understanding Biofilms and Chronic Infection

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Stabilizing a flexible interdomain hinge region harboring the SMB binding site drives uPAR into its closed conformation

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Crystal Structure of the Urokinase Receptor in a Ligand-Free Form

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Multiple barriers to recombination between divergent HIV-1 variants revealed by a dual-marker recombination assay

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. The discovery of bacterial biofilm in patients with muscle invasive bladder cancer

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Antibiotic susceptibility of cystic fibrosis lung microbiome members in a multispecies biofilm

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Bacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Early IL-2 treatment of mice with Pseudomonas aeruginosa pneumonia induced PMN-dominating response and reduced lung pathology

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Improving antibiotic treatment of bacterial biofilm by hyperbaric oxygen therapy: Not just hot air

    Research output: Contribution to journalReviewpeer-review

  • Christian Garde
  • Thomas Bjarnsholt
  • Michael Givskov
  • Tim Holm Jakobsen
  • Morten Hentzer
  • Anetta Claussen
  • Kim Sneppen
  • Jesper Ferkinghoff-Borg
  • Thomas Sams
View graph of relations
We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.
Original languageEnglish
JournalJournal of Molecular Biology
Volume396
Issue number4
Pages (from-to)849-57
Number of pages9
ISSN0022-2836
DOIs
Publication statusPublished - 5 Mar 2010

ID: 31052970