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Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique

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Basophils have been suggested to express low quantities of RNA, challenging the study of gene expression within these cells. However, the purification technique employed might have an impact on the quantity and quality of RNA purified from basophils. This chapter describes a method which gives an optimal RNA output using a TRIzol-based method in contrast to a commercial kit.

Original languageEnglish
JournalMethods in Molecular Biology
Volume2163
Pages (from-to)241-245
Number of pages5
ISSN1064-3745
DOIs
Publication statusPublished - 2020

ID: 61785213