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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Quantitative analysis of the cellular inflammatory response against biofilm bacteria in chronic wounds

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  1. Non-invasive measurement of reepithelialization and microvascularity of suction-blister wounds with benchmarking to histology

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  2. Consensus guidelines for the identification and treatment of biofilms in chronic nonhealing wounds

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  3. Impaired collagen synthesis in the rectum may be a molecular target in anastomotic leakage prophylaxis

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  4. Antiscarring pharmaceuticals: lost in translation?

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  1. The efficacy of topical agents used in wounds for managing chronic biofilm infections: A systematic review

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  2. Biofilms of Mycobacterium abscessus complex can be sensitized to antibiotics by disaggregation and oxygenation

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  3. Author Correction: Characterisation and localisation of the endocannabinoid system components in the adult human testis

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  4. Minimum information guideline for spectrofotometric and fluorometric methods to assess biofilm formation in microplates

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  5. Is pseudarthrosis after spinal instrumentation caused by a chronic infection?

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Chronic wounds are an important problem worldwide. These wounds are characterized by a persistent inflammatory stage associated with excessive accumulation and elevated cell activity of neutrophils, suggesting that there must be a persistent stimulus that attracts and recruits neutrophils to the wound. One such stimulus might be the presence of bacterial biofilms in chronic wounds. In the present study, biopsy specimens from chronic venous leg ulcers were investigated for the detection of bacteria using peptide nucleic acid-based fluorescence in situ hybridization (PNA-FISH) and confocal laser scanning microscopy. The bacteria in the wounds were often situated in large aggregates. To obtain a measure of the cellular inflammatory response against the bacteria in the chronic wounds, the amount of neutrophils accumulated at the site of infection was evaluated through differential neutrophil counting on the tissue sections from wounds containing either Pseudomonas aeruginosa or Staphylococcus aureus. The P. aeruginosa-containing wounds had significantly higher numbers of neutrophils accumulated compared with the S. aureus-containing wounds. These results are discussed in relation to the hypothesis that the presence of P. aeruginosa biofilms in chronic wounds may be one of the main factors leading to a persistent inflammatory response and impaired wound healing.
Original languageEnglish
JournalWound Repair and Regeneration
Volume19
Issue number3
Pages (from-to)387-91
Number of pages5
ISSN1067-1927
DOIs
Publication statusPublished - 2011

ID: 32661994