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Quantitation of the receptor for urokinase plasminogen activator by enzyme-linked immunosorbent assay

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  1. An IgY-based immunoassay to evaluate the biomarker potential of the Tannerella forsythia virulence factor karilysin in human saliva

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  2. The use of synthetic peptides for detection of anti-citrullinated protein antibodies in rheumatoid arthritis

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  3. A novel antihuman C3d monoclonal antibody with specificity to the C3d complement split product

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  4. Efficient evaluation of humoral immune responses by the use of serum pools

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  1. Prognostic utility of serum YKL-40 in patients with cervical cancer

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  2. Tumor cell MT1-MMP is dispensable for osteosarcoma tumor growth, bone degradation and lung metastasis

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  3. Chylomicronemia From GPIHBP1 Autoantibodies Successfully Treated With Rituximab: A Case Report

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  4. Chylomicronemia from GPIHBP1 autoantibodies

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Binding of the urokinase plasminogen activator (uPA) to a specific cell surface receptor (uPAR) plays a crucial role in proteolysis during tissue remodelling and cancer invasion. An immunosorbent assay for the quantitation of uPAR has now been developed. This assay is based on two monoclonal antibodies recognizing the non-ligand binding part of this receptor, and it detects both free and occupied uPAR, in contrast to ligand-binding assays used previously. In a variant of the assay, the occupied fraction of uPAR is selectively detected with a uPA antibody. To be used as a standard, a soluble variant of uPAR, suPAR, has been constructed by recombinant technique and the protein content of a purified suPAR standard preparation was determined by amino acid composition analysis. The sensitivity of the assay (0.6 ng uPAR/ml) is strong enough to measure uPAR in extracts of cultured cells and cancer tissue. Recent studies have shown that a high uPA level in tumor extracts is in some cancers associated with poor prognosis. The present assay will now allow similar prognostic studies of uPAR levels.

Original languageEnglish
JournalJournal of Immunological Methods
Volume167
Issue number1-2
Pages (from-to)91-101
Number of pages11
ISSN0022-1759
Publication statusPublished - 3 Jan 1994

    Research areas

  • Amino Acids, Animals, Antibodies, Monoclonal, CHO Cells, Carcinoma, Colonic Neoplasms, Cricetinae, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Recombinant Proteins, Tumor Cells, Cultured

ID: 46434998