Quantification of urinary etheno-DNA adducts by column-switching LC/APCI-MS/MS

Peter R Hillestrøm, Allan Weimann, Henrik E Poulsen

Abstract

Lipid peroxidation induced etheno-DNA adducts are promutagenic and have been suggested to play a causal role in the development of human cancers. Therefore, human biomonitoring of etheno-DNA adducts in urine has been suggested as a potential marker for oxidative stress-related DNA damage. For quantitative determination, a column-switching LC/APCI-MS/MS method was developed for simultaneous analysis of epsilonAde, epsilondC, and epsilondA in human urine. Quantitative validation parameters (precision, within-day repeatability, and between-day reproducibility) yielded satisfactory results below 10%. Limit of quantification for epsilonAde, epsilondC, and epsilondA was 5.3 fmol, 7.5 fmol, and 1.3 fmol on column, respectively. Mean urinary excretion rates of a six healthy volunteers were 45.8 pmol epsilonAde/24 h, 96.8 pmol epsilondC/24 h, and 18.1 pmol epsilondA/24 h. The demonstrated levels of performance suggest a future applicability of this method to studies of cancer and other diseases related to oxidative stress in humans. To our knowledge, this is the first method described that allows simultaneous determination of epsilonAde, epsilondC, and epsilondA in human urine samples.

Original languageEnglish
JournalJournal of the American Society for Mass Spectrometry
Volume17
Issue number4
Pages (from-to)605-610
Number of pages6
ISSN1044-0305
DOIs
Publication statusPublished - Apr 2006

Keywords

  • Biomarkers/chemistry
  • Chromatography, Liquid/methods
  • DNA Adducts/chemistry
  • DNA Damage
  • Humans
  • Lipid Peroxidation
  • Mass Spectrometry/methods
  • Oxidative Stress

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