Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Quantification of cell density in rat Achilles tendon: development and application of a new method

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Early reversal cells in adult human bone remodeling: osteoblastic nature, catabolic functions and interactions with osteoclasts

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Caveolae in fibroblast-like synoviocytes: static structures associated with vimentin-based intermediate filaments

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. The effects of freezing, storage, and thawing on cell compartment integrity and ultrastructure.

    Research output: Contribution to journalJournal articleResearch

  1. Maintenance of muscle strength following a one-year resistance training program in older adults

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The influence of prolonged strength training upon muscle and fat in healthy and chronically diseased older adults

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Associations between shoulder symptoms and concomitant pathology in patients with traumatic supraspinatus tears

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The effect of 4 months exercise training on systemic biomarkers of cartilage and bone turnover in hip osteoarthritis patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Early development of tendinopathy in humans: Sequence of pathological changes in structure and tissue turnover signaling

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Increased tendon cell nuclei density (TCND) has been proposed to induce tendon mechanical adaptations. However, it is unknown whether TCND is increased in tendon tissue after mechanical loading and whether such an increase can be quantified in a reliable manner. The aim of this study was to develop a reliable method for quantification of TCND and to investigate potential changes in TCND in rat Achilles tendons in response to 12 weeks of running. Eight adult male Sprague-Dawley rats ran (RUN) on a treadmill with 10° incline, 1 h/day, 5 days/wk (17-20 m/min) for 12 weeks (which improved tendon mechanical properties) and were compared with 11 control rats (SED). Tissue-Tek-embedded cryosections (10 µm) from the mid region of the Achilles tendon were cut longitudinally on a cryostat. Sections were stained with alcian blue and picrosirius red. One blinded investigator counted the number of tendon cell nuclei 2-3 times in three separate regions of the mid longitudinal tendon sections with fields of 390 μm × 280 μm. Unpaired t tests were used for the statistical analysis (mean ± SE). Typical Error % for replicate counts was 5.5 and 14 % coefficient of variation for the three regions. There was no difference in TCND between running rats versus control rats (nuclei per image (≈105 μm2): RUN, 152 ± 9; SED, 146 ± 8, p = 0.642). This new method provided reproducible quantification of TCND. There was no difference in TCND despite improvements in tendon mechanics, which suggests that cell number is not a major cause for altered tendon mechanical properties with loading.

Original languageEnglish
JournalHistochemistry and Cell Biology
Volume147
Issue number1
Pages (from-to)97-102
Number of pages6
ISSN0948-6143
DOIs
Publication statusPublished - Jan 2017

    Research areas

  • Achilles Tendon, Animals, Cell Count, Cell Nucleus, Male, Rats, Rats, Sprague-Dawley, Tissue Embedding, Journal Article

ID: 52334008