Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Pubertal development in healthy children is mirrored by DNA methylation patterns in peripheral blood

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Body mass index in young men and risk of inflammatory bowel disease through adult life: A population-based Danish cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Leukocyte telomere length is associated with elevated plasma glucose and HbA1c in young healthy men independent of birth weight

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Antibiotic use during pregnancy and childhood overweight: A population-based nationwide cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Multidimensional diffusion MRI with spectrally modulated gradients reveals unprecedented microstructural detail

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Large-scale GWAS reveals insights into the genetic architecture of same-sex sexual behavior

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Characterization of Human Adrenal Steroidogenesis during Fetal Development

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Populations, decreasing fertility, and reproductive health

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Puberty marks numerous physiological processes which are initiated by central activation of the hypothalamic-pituitary-gonadal axis, followed by development of secondary sexual characteristics. To a large extent, pubertal timing is heritable, but current knowledge of genetic polymorphisms only explains few months in the large inter-individual variation in the timing of puberty. We have analysed longitudinal genome-wide changes in DNA methylation in peripheral blood samples (n = 102) obtained from 51 healthy children before and after pubertal onset. We show that changes in single methylation sites are tightly associated with physiological pubertal transition and altered reproductive hormone levels. These methylation sites cluster in and around genes enriched for biological functions related to pubertal development. Importantly, we identified that methylation of the genomic region containing the promoter of TRIP6 was co-ordinately regulated as a function of pubertal development. In accordance, immunohistochemistry identified TRIP6 in adult, but not pre-pubertal, testicular Leydig cells and circulating TRIP6 levels doubled during puberty. Using elastic net prediction models, methylation patterns predicted pubertal development more accurately than chronological age. We demonstrate for the first time that pubertal attainment of secondary sexual characteristics is mirrored by changes in DNA methylation patterns in peripheral blood. Thus, modulations of the epigenome seem involved in regulation of the individual pubertal timing.

Original languageEnglish
JournalScientific Reports
Volume6
Pages (from-to)28657
ISSN2045-2322
DOIs
Publication statusPublished - 28 Jun 2016

    Research areas

  • Journal Article

ID: 49277387