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Proteomic Alterations in Follicular Fluid of Human Small Antral Follicles Collected from Polycystic Ovaries-A Pilot Study

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  1. Concentrations of oocyte secreted GDF9 and BMP15 decrease with MII transition during human IVM

    Research output: Contribution to journalJournal articlepeer-review

  2. Organotypic Culture of Testicular Tissue from Infant Boys with Cryptorchidism

    Research output: Contribution to journalJournal articlepeer-review

  3. Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries

    Research output: Contribution to journalJournal articlepeer-review

  4. Ovulation patterns affect the offspring sex ratios and change with the women's age

    Research output: Contribution to journalJournal articlepeer-review

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Polycystic ovaries (PCO) contain antral follicles that arrest growing around 3-11 mm in diameter, perturbing the dominant follicle's selection and the subsequent ovulatory process. Proteomic alterations of PCO follicular fluid (FF) (i.e., microenvironment in which the oocyte develops until ovulation) have been studied from large follicles in connection with oocyte pickup during ovarian stimulation. The present study aimed to detect proteomic alterations in FF from unstimulated human small antral follicles (hSAF) obtained from PCO. After performing deep-sequencing label-free proteomics on 10 PCO and 10 non-PCO FF samples from unstimulated hSAF (4.6-9.8 mm), 1436 proteins were identified, of which 115 were dysregulated in PCO FF samples. Pathways and processes related to the immune system, inflammation, and oxidative stress appeared to be upregulated in PCO, while extracellular matrix receptors interactions, the collagens-containing extracellular matrix, and the regulation of signaling were downregulated. The secreted proteins SFRP1, THBS4, and C1QC significantly decreased their expression in PCO FF, and this downregulation was suggested to affect future oocyte competence. In conclusion, our study revealed, for the first time, evidence of proteomic alterations occurring in the FF of PCO hSAF that may be related to the dysfunction of follicular growth and subsequent oocyte competence.

Original languageEnglish
Article number391
JournalLife (Basel, Switzerland)
Volume12
Issue number3
ISSN2075-1729
DOIs
Publication statusPublished - 8 Mar 2022

ID: 79719145