Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Cyclic nucleotide phosphodiesterases (PDEs) and endothelial function in ischaemic stroke. A review

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. A switch-variant model integrates the functions of an autoimmune variant of the phosphatase PTPN22

    Research output: Contribution to journalReviewResearchpeer-review

  3. Phosphodiesterase 5 inhibition as a therapeutic target for ischemic stroke: A systematic review of preclinical studies

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Role of AMPK in regulation of LC3 lipidation as a marker of autophagy in skeletal muscle

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Fine-tuned ATP signals are acute mediators in osteocyte mechanotransduction

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Gut microbiota profile and selected plasma metabolites in type 1 diabetes without and with stratification by albuminuria

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Profiling of gut microbiota and plasma metabolites in persons with type 1 diabetes and kidney disease

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

  3. Cell populations and molecular biomarkers in blood and urine characterise nephropathy in type 1 diabetes

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

  4. Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Brian T Weinert
  • Sebastian A Wagner
  • Heiko Horn
  • Peter Henriksen
  • Wenshe R Liu
  • Jesper V Olsen
  • Lars J Jensen
  • Chunaram Choudhary
View graph of relations

Posttranslational modification of proteins by acetylation and phosphorylation regulates most cellular processes in living organisms. Surprisingly, the evolutionary conservation of phosphorylated serine and threonine residues is only marginally higher than that of unmodified serines and threonines. With high-resolution mass spectrometry, we identified 1981 lysine acetylation sites in the proteome of Drosophila melanogaster. We used data sets of experimentally identified acetylation and phosphorylation sites in Drosophila and humans to analyze the evolutionary conservation of these modification sites between flies and humans. Site-level conservation analysis revealed that acetylation sites are highly conserved, significantly more so than phosphorylation sites. Furthermore, comparison of lysine conservation in Drosophila and humans with that in nematodes and zebrafish revealed that acetylated lysines were significantly more conserved than were nonacetylated lysines. Bioinformatics analysis using Gene Ontology terms suggested that the proteins with conserved acetylation control cellular processes such as protein translation, protein folding, DNA packaging, and mitochondrial metabolism. We found that acetylation of ubiquitin-conjugating E2 enzymes was evolutionarily conserved, and mutation of a conserved acetylation site impaired the function of the human E2 enzyme UBE2D3. This systems-level analysis of comparative posttranslational modification showed that acetylation is an anciently conserved modification and suggests that phosphorylation sites may have evolved faster than acetylation sites.

Original languageEnglish
JournalCellular Signalling
Volume4
Issue number183
Pages (from-to)ra48
ISSN0898-6568
DOIs
Publication statusPublished - 26 Jul 2011
Externally publishedYes

    Research areas

  • Acetylation, Animals, Computational Biology, DNA Primers/genetics, Drosophila Proteins/genetics, Drosophila melanogaster, Humans, Lysine/metabolism, Mass Spectrometry, Phosphorylation, Protein Processing, Post-Translational/genetics, Proteomics/methods, Species Specificity, Yeasts

ID: 54999368