Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

Jeppe Grøndahl Rasmussen; Ole Frøbert; Linda Pilgaard; Jens Kastrup; Ulf Simonsen; Vladimir Zachar; Trine Fink

doi:10.3109/14653249.2010.506505

Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

Jeppe Grøndahl Rasmussen, Ole Frøbert, Linda Pilgaard, Jens Kastrup, Ulf Simonsen, Vladimir Zachar, Trine Fink

90 Citations (Scopus)

Abstract

Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1% and 5% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.

Original language	English
Journal	Cytotherapy
Volume	13
Issue number	3
Pages (from-to)	318-28
Number of pages	11
ISSN	1465-3249
DOIs	https://doi.org/10.3109/14653249.2010.506505
Publication status	Published - 2011

Keywords

Adipose Tissue
Adult
Angiogenesis Inducing Agents
Animals
Apoptosis
Cell Culture Techniques
Cell Hypoxia
Cell Proliferation
Cell Survival
Cells, Cultured
Culture Media, Conditioned
Endothelium
Female
Gene Expression Regulation
Humans
Male
Middle Aged
Models, Biological
Neovascularization, Physiologic
Rats
Stem Cells
Trypsin

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10.3109/14653249.2010.506505

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title = "Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells",

abstract = "Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1\% and 5\% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.",

keywords = "Adipose Tissue, Adult, Angiogenesis Inducing Agents, Animals, Apoptosis, Cell Culture Techniques, Cell Hypoxia, Cell Proliferation, Cell Survival, Cells, Cultured, Culture Media, Conditioned, Endothelium, Female, Gene Expression Regulation, Humans, Male, Middle Aged, Models, Biological, Neovascularization, Physiologic, Rats, Stem Cells, Trypsin",

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year = "2011",

doi = "10.3109/14653249.2010.506505",

language = "English",

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pages = "318--28",

journal = "Cytotherapy",

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TY - JOUR

T1 - Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

AU - Rasmussen, Jeppe Grøndahl

AU - Frøbert, Ole

AU - Pilgaard, Linda

AU - Kastrup, Jens

AU - Simonsen, Ulf

AU - Zachar, Vladimir

AU - Fink, Trine

PY - 2011

Y1 - 2011

N2 - Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1% and 5% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.

AB - Transplantation of mesenchymal stromal cells (MSC), including adipose tissue-derived stem cells (ASC), is a promising option in the treatment of vascular disease. Short-term hypoxic culture of MSC augments secretion of anti-apoptotic and angiogenic cytokines. We hypothesized that prolonged hypoxic (1% and 5% oxygen) culture and trypsinization would augment ASC expression of anti-apoptotic and angiogenic cytokines and increase the angiogenic potential of ASC-conditioned media.

KW - Adipose Tissue

KW - Adult

KW - Angiogenesis Inducing Agents

KW - Animals

KW - Apoptosis

KW - Cell Culture Techniques

KW - Cell Hypoxia

KW - Cell Proliferation

KW - Cell Survival

KW - Cells, Cultured

KW - Culture Media, Conditioned

KW - Endothelium

KW - Female

KW - Gene Expression Regulation

KW - Humans

KW - Male

KW - Middle Aged

KW - Models, Biological

KW - Neovascularization, Physiologic

KW - Rats

KW - Stem Cells

KW - Trypsin

UR - https://www.scopus.com/pages/publications/79951690500

U2 - 10.3109/14653249.2010.506505

DO - 10.3109/14653249.2010.506505

M3 - Journal article

C2 - 20795759

SN - 1465-3249

VL - 13

SP - 318

EP - 328

JO - Cytotherapy

JF - Cytotherapy

IS - 3

ER -

Prolonged hypoxic culture and trypsinization increase the pro-angiogenic potential of human adipose tissue-derived stem cells

Abstract

Keywords

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