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Proliferation of germ cells and somatic cells in first trimester human embryonic gonads as indicated by S and S+G2+M phase fractions

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@article{ea2efdba262a4858abdd5e1667aa3f52,
title = "Proliferation of germ cells and somatic cells in first trimester human embryonic gonads as indicated by S and S+G2+M phase fractions",
abstract = "The number of germ cells and somatic cells in human embryonic and foetal gonads has previously been estimated by stereological methods, which are time- and labour-consuming with little information concerning cell proliferation. Here, we studied whether flow cytometry could be applied as an easier method, also enabling estimation of the fraction of cells in S or S+G(2)+M (SG(2) M) cell-cycle phases as indicators of cell proliferation.",
keywords = "Alkaline Phosphatase, Cell Proliferation, Cell Separation, Female, Flow Cytometry, Fluorescent Dyes, Germ Cells, Gonads, Humans, Immunomagnetic Separation, Male, Pregnancy, Pregnancy Trimester, First, Proto-Oncogene Proteins c-kit, U937 Cells",
author = "S{\o}rensen, {K P} and Lutterodt, {M C} and Mamsen, {L S} and Byskov, {A G} and Larsen, {J{\o}rgen Knud}",
note = "{\textcopyright} 2011 Blackwell Publishing Ltd.",
year = "2011",
doi = "10.1111/j.1365-2184.2011.00748.x",
language = "English",
volume = "44",
pages = "224--33",
journal = "Cell Proliferation",
issn = "0960-7722",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Proliferation of germ cells and somatic cells in first trimester human embryonic gonads as indicated by S and S+G2+M phase fractions

AU - Sørensen, K P

AU - Lutterodt, M C

AU - Mamsen, L S

AU - Byskov, A G

AU - Larsen, Jørgen Knud

N1 - © 2011 Blackwell Publishing Ltd.

PY - 2011

Y1 - 2011

N2 - The number of germ cells and somatic cells in human embryonic and foetal gonads has previously been estimated by stereological methods, which are time- and labour-consuming with little information concerning cell proliferation. Here, we studied whether flow cytometry could be applied as an easier method, also enabling estimation of the fraction of cells in S or S+G(2)+M (SG(2) M) cell-cycle phases as indicators of cell proliferation.

AB - The number of germ cells and somatic cells in human embryonic and foetal gonads has previously been estimated by stereological methods, which are time- and labour-consuming with little information concerning cell proliferation. Here, we studied whether flow cytometry could be applied as an easier method, also enabling estimation of the fraction of cells in S or S+G(2)+M (SG(2) M) cell-cycle phases as indicators of cell proliferation.

KW - Alkaline Phosphatase

KW - Cell Proliferation

KW - Cell Separation

KW - Female

KW - Flow Cytometry

KW - Fluorescent Dyes

KW - Germ Cells

KW - Gonads

KW - Humans

KW - Immunomagnetic Separation

KW - Male

KW - Pregnancy

KW - Pregnancy Trimester, First

KW - Proto-Oncogene Proteins c-kit

KW - U937 Cells

U2 - 10.1111/j.1365-2184.2011.00748.x

DO - 10.1111/j.1365-2184.2011.00748.x

M3 - Journal article

C2 - 21535263

VL - 44

SP - 224

EP - 233

JO - Cell Proliferation

JF - Cell Proliferation

SN - 0960-7722

IS - 3

ER -

ID: 33123294