Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Progressive changes in human follicular fluid composition over the course of ovulation: quantitative proteomic analyses

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Human granulosa cells function as innate immune cells executing an inflammatory reaction during ovulation: a microarray analysis

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Identification of circulating small non-coding RNAs in relation to male subfertility and reproductive hormones

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Regulation of the β-cell inflammasome and contribution to stress-induced cellular dysfunction and apoptosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Experimental models of testicular development and function using human tissue and cells

    Research output: Contribution to journalReviewResearchpeer-review

  1. A distinctive epigenetic ageing profile in human granulosa cells

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Postnatal germ cell development in cryptorchid boys

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Review of injection techniques for spermatogonial stem cell transplantation

    Research output: Contribution to journalReviewResearchpeer-review

  4. Ovarian cortical follicle density in infertile women with low anti-Müllerian hormone

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Gene Expression in Granulosa Cells From Small Antral Follicles From Women With or Without Polycystic Ovaries

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Follicular fluid (FF) acts as a vehicle for paracrine signalling between somatic cells of the follicle and the oocyte. To investigate changes in the protein composition of FF during ovulation, we conducted a prospective cohort study including 25 women undergoing fertility treatment. Follicular fluid was aspirated either before or 12, 17, 32 or 36 h after induction of ovulation (five patients per time point). Liquid chromatography-mass spectrometry was used to identify and quantify FF proteins. In total, 400 proteins were identified and the levels of 40 proteins changed significantly across ovulation, evaluated by analysis of covariance (adjusted p < 0.05) and on-off expression patterns. The majority peaked after 12-17 h, e.g., AREG (p < 0.0001), TNFAIP6 (p < 0.0001), and LDHB (p = 0.0316), while some increased to peak after 36 h e.g., ACPP (p < 0.0001), TIMP1 (p < 0.0001) and SERPINE1 (p = 0.0002). Collectively, this study highlights proteins and pathways of importance for ovulation and oocyte competence in humans.

Original languageEnglish
JournalMolecular and Cellular Endocrinology
Volume495
Pages (from-to)110522
ISSN0303-7207
DOIs
Publication statusPublished - 15 Sep 2019

ID: 59045181