Prognostic role of Ki-67 in glioblastomas excluding contribution from non-neoplastic cells

Rikke H Dahlrot, Julie A Bangsø, Jeanette K Petersen, Ann Mari Rosager, Mia D Sørensen, Guido Reifenberger, Steinbjørn Hansen, Bjarne W Kristensen

28 Citations (Scopus)

Abstract

Survival of glioblastoma patients varies and prognostic markers are important in the clinical setting. With digital pathology and improved immunohistochemical multiplexing becoming a part of daily diagnostics, we investigated the prognostic value of the Ki-67 labelling index (LI) in glioblastomas more precisely than previously by excluding proliferation in non-tumor cells from the analysis. We investigated the Ki-67 LI in a well-annotated population-based glioblastoma patient cohort (178 IDH-wildtype, 3 IDH-mutated). Ki-67 was identified in full tumor sections with automated digital image analysis and the contribution from non-tumor cells was excluded using quantitative double-immunohistochemistry. For comparison of the Ki-67 LI between WHO grades (II-IV), 9 IDH-mutated diffuse astrocytomas and 9 IDH-mutated anaplastic astrocytomas were stained. Median Ki-67 LI increased with increasing WHO grade (median 2.7%, 6.4% and 27.5%). There was no difference in median Ki-67 LI between IDH-mutated and IDH-wildtype glioblastomas (p = 0.9) and Ki-67 LI was not associated with survival in glioblastomas in neither univariate (p = 0.9) nor multivariate analysis including MGMT promoter methylation status and excluding IDH-mutated glioblastomas (p = 0.2). Ki-67 may be of value in the differential diagnostic setting, but it must not be over-interpreted in the clinico-pathological context.

Original languageEnglish
Article number17918
JournalScientific Reports
Volume11
Issue number1
Pages (from-to)17918
ISSN2045-2322
DOIs
Publication statusPublished - 9 Sept 2021

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor/metabolism
  • Cohort Studies
  • Female
  • Glioblastoma/metabolism
  • Humans
  • Ki-67 Antigen/metabolism
  • Male
  • Middle Aged
  • Neoplasm Grading
  • Prognosis

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