Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes

E J Kovacs; B Brock; I E Silber; J E Neuman

Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes

E J Kovacs, B Brock, I E Silber, J E Neuman

14 Citations (Scopus)

Abstract

OBJECTIVE: To assess the production of fibrogenic cytokines by interleukin-2 (IL-2)-stimulated peripheral blood leukocytes and to examine their ability to stimulate the production of connective tissue.

METHODS: Culture medium from human peripheral blood leukocytes incubated with or without IL-2 was tested for induction of fibroblast proliferation, collagen synthesis, and expression of cytokine genes.

RESULTS: Supernatants from IL-2-treated peripheral blood leukocytes induced six times more fibroblast proliferation than medium from leukocytes cultured without IL-2. The expression of type I procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment.

CONCLUSION: Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy.

Original language	English
Journal	Obstetrics and Gynecology
Volume	82
Issue number	1
Pages (from-to)	29-36
Number of pages	8
ISSN	0029-7844
Publication status	Published - Jul 1993
Externally published	Yes

Keywords

Animals
Cell Division
Cells, Cultured
Collagen
Connective Tissue Cells
Cytokines
Fibroblasts
Fibronectins
Gene Expression
Humans
Interleukin-1
Interleukin-2
Killer Cells, Lymphokine-Activated
Leukocytes
Platelet-Derived Growth Factor
Procollagen
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-sis
RNA, Messenger
Rats
Rats, Sprague-Dawley
Tissue Adhesions
Transforming Growth Factor alpha
Transforming Growth Factor beta
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

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Kovacs, E. J., Brock, B., Silber, I. E., & Neuman, J. E. (1993). Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes. Obstetrics and Gynecology, 82(1), 29-36. http://Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes

Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes. / Kovacs, E J; Brock, B; Silber, I E et al.
In: Obstetrics and Gynecology, Vol. 82, No. 1, 07.1993, p. 29-36.

Research output: Contribution to journal › Journal article › Research › peer-review

Kovacs, EJ, Brock, B, Silber, IE & Neuman, JE 1993, 'Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes', Obstetrics and Gynecology, vol. 82, no. 1, pp. 29-36. <http://Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes>

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title = "Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes",

abstract = "OBJECTIVE: To assess the production of fibrogenic cytokines by interleukin-2 (IL-2)-stimulated peripheral blood leukocytes and to examine their ability to stimulate the production of connective tissue.METHODS: Culture medium from human peripheral blood leukocytes incubated with or without IL-2 was tested for induction of fibroblast proliferation, collagen synthesis, and expression of cytokine genes.RESULTS: Supernatants from IL-2-treated peripheral blood leukocytes induced six times more fibroblast proliferation than medium from leukocytes cultured without IL-2. The expression of type I procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment.CONCLUSION: Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy.",

keywords = "Animals, Cell Division, Cells, Cultured, Collagen, Connective Tissue Cells, Cytokines, Fibroblasts, Fibronectins, Gene Expression, Humans, Interleukin-1, Interleukin-2, Killer Cells, Lymphokine-Activated, Leukocytes, Platelet-Derived Growth Factor, Procollagen, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-sis, RNA, Messenger, Rats, Rats, Sprague-Dawley, Tissue Adhesions, Transforming Growth Factor alpha, Transforming Growth Factor beta, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.",

author = "Kovacs, \{E J\} and B Brock and Silber, \{I E\} and Neuman, \{J E\}",

year = "1993",

month = jul,

language = "English",

volume = "82",

pages = "29--36",

journal = "Obstetrics and Gynecology",

issn = "0029-7844",

publisher = "Lippincott Williams and Wilkins",

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T1 - Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes

T2 - expression of transforming growth factor-beta and platelet-derived growth factor B chain genes

AU - Kovacs, E J

AU - Brock, B

AU - Silber, I E

AU - Neuman, J E

PY - 1993/7

Y1 - 1993/7

N2 - OBJECTIVE: To assess the production of fibrogenic cytokines by interleukin-2 (IL-2)-stimulated peripheral blood leukocytes and to examine their ability to stimulate the production of connective tissue.METHODS: Culture medium from human peripheral blood leukocytes incubated with or without IL-2 was tested for induction of fibroblast proliferation, collagen synthesis, and expression of cytokine genes.RESULTS: Supernatants from IL-2-treated peripheral blood leukocytes induced six times more fibroblast proliferation than medium from leukocytes cultured without IL-2. The expression of type I procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment.CONCLUSION: Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy.

AB - OBJECTIVE: To assess the production of fibrogenic cytokines by interleukin-2 (IL-2)-stimulated peripheral blood leukocytes and to examine their ability to stimulate the production of connective tissue.METHODS: Culture medium from human peripheral blood leukocytes incubated with or without IL-2 was tested for induction of fibroblast proliferation, collagen synthesis, and expression of cytokine genes.RESULTS: Supernatants from IL-2-treated peripheral blood leukocytes induced six times more fibroblast proliferation than medium from leukocytes cultured without IL-2. The expression of type I procollagen and fibronectin messenger RNAs was increased in human fibroblasts in response to leukocyte supernatants. Unstimulated leukocytes expressed minimal levels of transforming growth factor-beta or platelet-derived growth factor B chain messenger RNAs, but could be greatly enhanced by IL-2 treatment.CONCLUSION: Mediators that induce connective tissue production are secreted by IL-2-treated peripheral blood leukocytes. These cytokines may be responsible, in part, for the stimulation of abdominal adhesions in patients receiving intraperitoneal immunotherapy.

KW - Animals

KW - Cell Division

KW - Cells, Cultured

KW - Collagen

KW - Connective Tissue Cells

KW - Cytokines

KW - Fibroblasts

KW - Fibronectins

KW - Gene Expression

KW - Humans

KW - Interleukin-1

KW - Interleukin-2

KW - Killer Cells, Lymphokine-Activated

KW - Leukocytes

KW - Platelet-Derived Growth Factor

KW - Procollagen

KW - Proto-Oncogene Proteins

KW - Proto-Oncogene Proteins c-sis

KW - RNA, Messenger

KW - Rats

KW - Rats, Sprague-Dawley

KW - Tissue Adhesions

KW - Transforming Growth Factor alpha

KW - Transforming Growth Factor beta

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

UR - https://www.scopus.com/pages/publications/0027159875

M3 - Journal article

C2 - 8515922

SN - 0029-7844

VL - 82

SP - 29

EP - 36

JO - Obstetrics and Gynecology

JF - Obstetrics and Gynecology

IS - 1

ER -

Production of fibrogenic cytokines by interleukin-2-treated peripheral blood leukocytes: expression of transforming growth factor-beta and platelet-derived growth factor B chain genes

Abstract

Keywords

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