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Processing-independent proANP measurement for low concentrations in plasma: reference intervals and effect of body mass index and plasma glucose

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@article{110b827f4a5e40e69c5efdc55ff7549c,
title = "Processing-independent proANP measurement for low concentrations in plasma: reference intervals and effect of body mass index and plasma glucose",
abstract = "BACKGROUND: Decreased concentrations of pro-atrial-derived natriuretic peptides (proABP) in plasma have been associated with obesity and suggested as a predictor of type 2 diabetes. However, assays for measuring proANP are generally aimed to quantitate higher concentrations of proANP associated with cardiac disease. Therefore, we aimed to measure plasma proANP concentrations in a non-obese Scandinavian reference material and evaluate potential associations of plasma proANP with body mass index (BMI) and plasma glucose, respectively.METHODS: We report an optimized processing-independent assay (PIA) for proANP in the lower concentration range. The assay was optimized by raising the amount of radioactive tracer and modifying the mixing ratio of resuspended plasma and buffer. Blood samples from a Scandinavian plasma cohort of 693 healthy subjects were then analyzed and age and gender-specific reference intervals were determined.RESULTS: Simple linear regression analyses of proANP and both BMI and plasma glucose in fasting subjects displayed insignificant associations. Multiple regression analyses supported these findings. However, a higher median plasma concentration of proANP was noted among women <50 years compared to men, whereas no gender-specific differences were seen in other age groups.CONCLUSIONS: Our results show that in a healthy non-obese population, BMI and plasma glucose in fasting subjects do not affect plasma proANP concentrations. Our method should be considered for future studies on low proANP concentration studies, e.g. in obesity and diabetes.",
keywords = "Aged, Atrial Natriuretic Factor, Blood Chemical Analysis, Blood Glucose, Body Mass Index, Female, Humans, Linear Models, Male, Middle Aged, Reference Values, Journal Article",
author = "Mark, {Peter D} and Ingrid Hunter and Dijana Terzic and Goetze, {Jens P}",
year = "2018",
doi = "10.1515/cclm-2017-0309",
language = "English",
volume = "56",
pages = "132--137",
journal = "Clinical Chemistry and Laboratory Medicine",
issn = "1434-6621",
publisher = "Walter/de Gruyter GmbH & Co. KG",
number = "1",

}

RIS

TY - JOUR

T1 - Processing-independent proANP measurement for low concentrations in plasma

T2 - reference intervals and effect of body mass index and plasma glucose

AU - Mark, Peter D

AU - Hunter, Ingrid

AU - Terzic, Dijana

AU - Goetze, Jens P

PY - 2018

Y1 - 2018

N2 - BACKGROUND: Decreased concentrations of pro-atrial-derived natriuretic peptides (proABP) in plasma have been associated with obesity and suggested as a predictor of type 2 diabetes. However, assays for measuring proANP are generally aimed to quantitate higher concentrations of proANP associated with cardiac disease. Therefore, we aimed to measure plasma proANP concentrations in a non-obese Scandinavian reference material and evaluate potential associations of plasma proANP with body mass index (BMI) and plasma glucose, respectively.METHODS: We report an optimized processing-independent assay (PIA) for proANP in the lower concentration range. The assay was optimized by raising the amount of radioactive tracer and modifying the mixing ratio of resuspended plasma and buffer. Blood samples from a Scandinavian plasma cohort of 693 healthy subjects were then analyzed and age and gender-specific reference intervals were determined.RESULTS: Simple linear regression analyses of proANP and both BMI and plasma glucose in fasting subjects displayed insignificant associations. Multiple regression analyses supported these findings. However, a higher median plasma concentration of proANP was noted among women <50 years compared to men, whereas no gender-specific differences were seen in other age groups.CONCLUSIONS: Our results show that in a healthy non-obese population, BMI and plasma glucose in fasting subjects do not affect plasma proANP concentrations. Our method should be considered for future studies on low proANP concentration studies, e.g. in obesity and diabetes.

AB - BACKGROUND: Decreased concentrations of pro-atrial-derived natriuretic peptides (proABP) in plasma have been associated with obesity and suggested as a predictor of type 2 diabetes. However, assays for measuring proANP are generally aimed to quantitate higher concentrations of proANP associated with cardiac disease. Therefore, we aimed to measure plasma proANP concentrations in a non-obese Scandinavian reference material and evaluate potential associations of plasma proANP with body mass index (BMI) and plasma glucose, respectively.METHODS: We report an optimized processing-independent assay (PIA) for proANP in the lower concentration range. The assay was optimized by raising the amount of radioactive tracer and modifying the mixing ratio of resuspended plasma and buffer. Blood samples from a Scandinavian plasma cohort of 693 healthy subjects were then analyzed and age and gender-specific reference intervals were determined.RESULTS: Simple linear regression analyses of proANP and both BMI and plasma glucose in fasting subjects displayed insignificant associations. Multiple regression analyses supported these findings. However, a higher median plasma concentration of proANP was noted among women <50 years compared to men, whereas no gender-specific differences were seen in other age groups.CONCLUSIONS: Our results show that in a healthy non-obese population, BMI and plasma glucose in fasting subjects do not affect plasma proANP concentrations. Our method should be considered for future studies on low proANP concentration studies, e.g. in obesity and diabetes.

KW - Aged

KW - Atrial Natriuretic Factor

KW - Blood Chemical Analysis

KW - Blood Glucose

KW - Body Mass Index

KW - Female

KW - Humans

KW - Linear Models

KW - Male

KW - Middle Aged

KW - Reference Values

KW - Journal Article

U2 - 10.1515/cclm-2017-0309

DO - 10.1515/cclm-2017-0309

M3 - Journal article

VL - 56

SP - 132

EP - 137

JO - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

SN - 1434-6621

IS - 1

ER -

ID: 52184043