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Processing-independent analysis of peptide hormones and prohormones in plasma

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@article{551051e4b4ae4c87a1a29d3e70a2f133,
title = "Processing-independent analysis of peptide hormones and prohormones in plasma",
abstract = "Peptide hormones are post-translationally matured before they reach a structure in which they can fulfill their biological functions. The prohormone processing may encompass a variety of endoproteolytic cleavages, N- and C-terminal trimmings, and amino acid derivatizations. The same prohormone can be variably processed in different cell types and, in addition, diseased cells often change the processing of a given precursor. The translational process is often either increased or decreased in diseased cells, which renders the ensuing modifications of the prohormone incomplete. Consequently, a variable mixture of precursors and processing-intermediates accumulates in plasma. In order to exploit disturbed posttranslational processing for diagnostic use and at the same time provide an accurate measure of the translational product, a simple analytical principle named {"}processing-independent analysis{"} (PIA) was designed. PIA-methods quantitate the total mRNA product irrespective of the degree of processing. PIA-methods have now been developed for a number of prohormones and proteins, and their diagnostic potential appears promising in diagnosis of cardiovascular disease and in several malignancies.",
keywords = "Amino Acid Sequence, Gene Expression, Hormones, Humans, Molecular Sequence Data, Peptides, Protein Processing, Post-Translational, Sequence Homology, Amino Acid",
author = "Goetze, {Jens Peter} and Ingrid Hunter and Lippert, {Solvej Koelvraa} and Linda Bardram and Rehfeld, {Jens Frederik}",
year = "2012",
language = "English",
volume = "17",
pages = "1804--15",
journal = "Frontiers in Bioscience",
issn = "1093-9946",
publisher = "Frontiers in Bioscience",

}

RIS

TY - JOUR

T1 - Processing-independent analysis of peptide hormones and prohormones in plasma

AU - Goetze, Jens Peter

AU - Hunter, Ingrid

AU - Lippert, Solvej Koelvraa

AU - Bardram, Linda

AU - Rehfeld, Jens Frederik

PY - 2012

Y1 - 2012

N2 - Peptide hormones are post-translationally matured before they reach a structure in which they can fulfill their biological functions. The prohormone processing may encompass a variety of endoproteolytic cleavages, N- and C-terminal trimmings, and amino acid derivatizations. The same prohormone can be variably processed in different cell types and, in addition, diseased cells often change the processing of a given precursor. The translational process is often either increased or decreased in diseased cells, which renders the ensuing modifications of the prohormone incomplete. Consequently, a variable mixture of precursors and processing-intermediates accumulates in plasma. In order to exploit disturbed posttranslational processing for diagnostic use and at the same time provide an accurate measure of the translational product, a simple analytical principle named "processing-independent analysis" (PIA) was designed. PIA-methods quantitate the total mRNA product irrespective of the degree of processing. PIA-methods have now been developed for a number of prohormones and proteins, and their diagnostic potential appears promising in diagnosis of cardiovascular disease and in several malignancies.

AB - Peptide hormones are post-translationally matured before they reach a structure in which they can fulfill their biological functions. The prohormone processing may encompass a variety of endoproteolytic cleavages, N- and C-terminal trimmings, and amino acid derivatizations. The same prohormone can be variably processed in different cell types and, in addition, diseased cells often change the processing of a given precursor. The translational process is often either increased or decreased in diseased cells, which renders the ensuing modifications of the prohormone incomplete. Consequently, a variable mixture of precursors and processing-intermediates accumulates in plasma. In order to exploit disturbed posttranslational processing for diagnostic use and at the same time provide an accurate measure of the translational product, a simple analytical principle named "processing-independent analysis" (PIA) was designed. PIA-methods quantitate the total mRNA product irrespective of the degree of processing. PIA-methods have now been developed for a number of prohormones and proteins, and their diagnostic potential appears promising in diagnosis of cardiovascular disease and in several malignancies.

KW - Amino Acid Sequence

KW - Gene Expression

KW - Hormones

KW - Humans

KW - Molecular Sequence Data

KW - Peptides

KW - Protein Processing, Post-Translational

KW - Sequence Homology, Amino Acid

M3 - Journal article

VL - 17

SP - 1804

EP - 1815

JO - Frontiers in Bioscience

JF - Frontiers in Bioscience

SN - 1093-9946

ER -

ID: 36731322