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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Processing-independent analysis of peptide hormones and prohormones in plasma

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Peptide hormones are post-translationally matured before they reach a structure in which they can fulfill their biological functions. The prohormone processing may encompass a variety of endoproteolytic cleavages, N- and C-terminal trimmings, and amino acid derivatizations. The same prohormone can be variably processed in different cell types and, in addition, diseased cells often change the processing of a given precursor. The translational process is often either increased or decreased in diseased cells, which renders the ensuing modifications of the prohormone incomplete. Consequently, a variable mixture of precursors and processing-intermediates accumulates in plasma. In order to exploit disturbed posttranslational processing for diagnostic use and at the same time provide an accurate measure of the translational product, a simple analytical principle named "processing-independent analysis" (PIA) was designed. PIA-methods quantitate the total mRNA product irrespective of the degree of processing. PIA-methods have now been developed for a number of prohormones and proteins, and their diagnostic potential appears promising in diagnosis of cardiovascular disease and in several malignancies.
Original languageEnglish
JournalFrontiers in bioscience : a journal and virtual library
Volume17
Pages (from-to)1804-15
Number of pages12
Publication statusPublished - 2012

    Research areas

  • Amino Acid Sequence, Gene Expression, Hormones, Humans, Molecular Sequence Data, Peptides, Protein Processing, Post-Translational, Sequence Homology, Amino Acid

ID: 36731322