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Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome

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  1. Cryptorchidism, gonocyte development, and the risks of germ cell malignancy and infertility: A systematic review

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  2. Impaired serum inhibin-B and number of germ cells in boys with cryptorchidism following heavily gestational maternal smoking

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  3. Outcomes of biliary atresia in the Nordic countries - a multicenter study of 158 patients during 2005-2016

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  1. Laparoscopy to Assist Surgical Decisions Related to Necrotizing Enterocolitis in Preterm Neonates

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  2. Gonocyte transformation in congenital undescended testes: what is the role of inhibin-B in cell death?

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  3. Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys

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  4. Propagation of Spermatogonial Stem Cell-Like Cells From Infant Boys

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INTRODUCTION: Neonatal testicular germ cells/gonocytes, transform into stem cells for spermatogenesis during 'minipuberty', driving change in timing of surgery. This study examined gonocyte transformation in cryptorchid testes in children ≤18 months of age with unilateral, bilateral undescended testes (UDT), complete or partial androgen insensitivity syndrome (CAIS, PAIS) [3,4].

MATERIAL AND METHODS: Testicular biopsies were taken from patients with unilateral or bilateral UDT, PAIS or CAIS, aged 10 days-18 months. These testicular sections underwent immunohistochemistry with antibodies (Oct4, Ki67, C-Kit, Sox9) followed by confocal imaging, cell counting and statistical analysis.

RESULTS: Both Sertoli cells/tubule and germ cells (GC)/tubule decreased with age, and % empty tubules (no GC) increased with age but with no significant differences between patient groups. Oct4+ germ cells/tubule decreased with age. There are some GCs and Sertoli cells proliferating during the first year and most proliferating Oct4+ germ cells (Oct4+/Ki67+) were located off tubular basement membrane.

CONCLUSION: Our study showed that Oct4 expression gradually decreased after minipuberty and transformation into spermatogonia. Germ cells and Sertoli cells undergo mitosis during the first 12 months although not abundantly. We propose that Oct4+ gonocyte transformation into spermatogonia via proliferation and migration to the basement membrane may be delayed in UDT.

Original languageEnglish
JournalJournal of Pediatric Surgery
Volume54
Issue number8
Pages (from-to)1654-1659
Number of pages6
ISSN0022-3468
DOIs
Publication statusPublished - 1 Aug 2019

    Research areas

  • Cryptorchidism, Germ cells, Gonocyte, Spermatogonium, Testicular cancer, Undescended testis, Cryptorchidism/pathology, Octamer Transcription Factor-3/metabolism, Spermatogenesis, Cell Count, Humans, Infant, Male, Androgen-Insensitivity Syndrome/pathology, Sertoli Cells/pathology, Basement Membrane/pathology, Testis/pathology, Spermatogonia/metabolism, Cell Differentiation, Infant, Newborn

ID: 56518908