Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

Helle Brander Eriksen; Andreas Petersen; Michael Pedersen; Søren Overballe-Petersen; Anders Rhod Larsen; Barbara Juliane Holzknecht

doi:10.1007/s10096-021-04344-w

Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

Helle Brander Eriksen, Andreas Petersen, Michael Pedersen, Søren Overballe-Petersen, Anders Rhod Larsen, Barbara Juliane Holzknecht

Abstract

We describe a case of recurrent catheter-related blood stream infections (BSI) with Staphylococcus aureus, in which the first isolate tested susceptible to penicillin, while subsequent isolates were resistant. Phenotypic susceptibility correlated with the absence/presence of the blaZ gene. The in vitro stability of penicillin resistance was investigated by subculturing single colonies. In two out of five colonies, phenotypical resistance was lost after a single subculture, which correlated with loss of the blaZ gene. This in vitro phenomenon probably resulted in a very major error in the microbiology report of the first BSI, where penicillin had been recommended as treatment.

Original language	English
Journal	European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Volume	41
Issue number	1
Pages (from-to)	163-167
Number of pages	5
ISSN	0934-9723
DOIs	https://doi.org/10.1007/s10096-021-04344-w
Publication status	Published - Jan 2022

Keywords

blaZ
In vitro susceptibility testing
PSSA PRSA
Staphylococcus aureus

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Eriksen, H. B., Petersen, A., Pedersen, M., Overballe-Petersen, S., Larsen, A. R., & Holzknecht, B. J. (2022). Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 41(1), 163-167. https://doi.org/10.1007/s10096-021-04344-w

Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. / Eriksen, Helle Brander; Petersen, Andreas; Pedersen, Michael et al.

In: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, Vol. 41, No. 1, 01.2022, p. 163-167.

Research output: Contribution to journal › Journal article › Research › peer-review

Eriksen, HB, Petersen, A, Pedersen, M, Overballe-Petersen, S, Larsen, AR & Holzknecht, BJ 2022, 'Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene', European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, vol. 41, no. 1, pp. 163-167. https://doi.org/10.1007/s10096-021-04344-w

Eriksen HB, Petersen A, Pedersen M, Overballe-Petersen S, Larsen AR, Holzknecht BJ. Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology. 2022 Jan;41(1):163-167. https://doi.org/10.1007/s10096-021-04344-w

Eriksen, Helle Brander ; Petersen, Andreas ; Pedersen, Michael et al. / Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene. In: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology. 2022 ; Vol. 41, No. 1. pp. 163-167.

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Possible misinterpretation of penicillin susceptibility in Staphylococcus aureus blood isolate due to in vitro loss of the blaZ gene

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