Polyclonal Peptide Antisera

Tina H Pihl, Kristin E Engelhart, Gunnar Houen*

*Corresponding author for this work

Abstract

Polyclonal antibodies are relatively easy to produce and may supplement monoclonal antibodies for some applications or even have some advantages.The choice of species for production of (peptide) antisera is based on practical considerations, including availability of immunogen (vaccine) and animals. Two major factors govern the production of antisera: the nature of adaptive immune responses, which take place over days/weeks and ethical guidelines for animal welfare.Here, simple procedures for immunization of mice, rabbits, sheep, goats, pigs, horses, and chickens are presented.

Original languageEnglish
Title of host publicationPeptide Antibodies
Number of pages5
Volume2821
PublisherSpringer
Publication date2024
Pages129-133
DOIs
Publication statusPublished - 2024
SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Animals
  • Immune Sera/chemistry
  • Mice
  • Rabbits
  • Peptides/immunology
  • Immunization
  • Horses/immunology
  • Sheep
  • Goats
  • Swine
  • Chickens/immunology

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