Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Pituitary porcine FSH, and recombinant bovine and human FSH differentially affect growth and relative abundances of mRNA transcripts of preantral and early developing antral follicles in goats

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

APA

Ferreira, A. C. A., Sá, N. A. R., Cadenas, J., Correia, H. H. V., Guerreiro, D. D., Alves, B. G., Lima, L. F., Celestino, J. J. H., Rodrigues, A. P. P. R., Gastal, E. L., & Figueiredo, J. R. (2020). Pituitary porcine FSH, and recombinant bovine and human FSH differentially affect growth and relative abundances of mRNA transcripts of preantral and early developing antral follicles in goats. Animal Reproduction Science, 219, 106461. [106461]. https://doi.org/10.1016/j.anireprosci.2020.106461

CBE

MLA

Vancouver

Author

Ferreira, Anna Clara A ; Sá, Naiza A R ; Cadenas, Jesús ; Correia, Hudson H V ; Guerreiro, Denise D ; Alves, Benner G ; Lima, Laritza F ; Celestino, Juliana J H ; Rodrigues, Ana Paula P R ; Gastal, Eduardo L ; Figueiredo, Jose R. / Pituitary porcine FSH, and recombinant bovine and human FSH differentially affect growth and relative abundances of mRNA transcripts of preantral and early developing antral follicles in goats. In: Animal Reproduction Science. 2020 ; Vol. 219. pp. 106461.

Bibtex

@article{a5b15fba02f3498dbbd030c956beb941,
title = "Pituitary porcine FSH, and recombinant bovine and human FSH differentially affect growth and relative abundances of mRNA transcripts of preantral and early developing antral follicles in goats",
abstract = "Three different sources of FSH (porcine pituitary, pFSH; recombinant bovine, rbFSH; and recombinant human, rhFSH) were compared during in vitro culture of preantral and early antral follicles of goats for 18 days. Treatments were: base medium supplemented with no FSH (control), 10, 50, or 100 mIU/mL pFSH (pFSH10, pFSH50, and pFSH100, respectively), 100 ng/mL rbFSH (rbFSH), and 50 mIU/mL rhFSH (rhFSH). There were evaluations of follicle morphology, antrum formation, growth rate, estradiol production, oocyte viability and chromatin configuration, and follicle wall relative abundance of mRNA transcript for MMP-9, TIMP-2, CYP17, CYP19A1, FSHR, Insulin-R, and BAX/BCL-2 ratio. Follicle degeneration rates were similar among all treatment groups at the end of culturing. When there were treatments with pFSH, however, there was a lesser (P < 0.05) percentage of intact follicles and estradiol production, and greater (P < 0.05) extrusion rates. Furthermore, with only pFSH10 (antral follicles) and pFSH100 (preantral and antral follicles) treatments, there was a lesser (P < 0.05) follicle growth. For preantral follicles, when there was addition of pFSH10, pFSH100, and rhFSH there was lesser (P < 0.05) oocyte meiotic resumption compared to control and rbFSH treatments. For antral follicles, when there were treatments with rhFSH and pFSH10 there was greater (P = 0.08 - P < 0.05) oocyte maturation. In conclusion, the source of FSH differentially affected gene expression, as indicated by mRNA abundances, and follicular dynamics of preantral and antral follicles in vitro. Addition of FSH during the in vitro culture improved the developmental outcomes only for antral follicles.",
keywords = "Antral follicles, FSH sources, Gene expression, Goat, Oocyte maturation, Preantral follicles",
author = "Ferreira, {Anna Clara A} and S{\'a}, {Naiza A R} and Jes{\'u}s Cadenas and Correia, {Hudson H V} and Guerreiro, {Denise D} and Alves, {Benner G} and Lima, {Laritza F} and Celestino, {Juliana J H} and Rodrigues, {Ana Paula P R} and Gastal, {Eduardo L} and Figueiredo, {Jose R}",
note = "Copyright {\textcopyright} 2020. Published by Elsevier B.V.",
year = "2020",
month = aug,
doi = "10.1016/j.anireprosci.2020.106461",
language = "English",
volume = "219",
pages = "106461",
journal = "Animal Reproduction Science",
issn = "0378-4320",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Pituitary porcine FSH, and recombinant bovine and human FSH differentially affect growth and relative abundances of mRNA transcripts of preantral and early developing antral follicles in goats

AU - Ferreira, Anna Clara A

AU - Sá, Naiza A R

AU - Cadenas, Jesús

AU - Correia, Hudson H V

AU - Guerreiro, Denise D

AU - Alves, Benner G

AU - Lima, Laritza F

AU - Celestino, Juliana J H

AU - Rodrigues, Ana Paula P R

AU - Gastal, Eduardo L

AU - Figueiredo, Jose R

N1 - Copyright © 2020. Published by Elsevier B.V.

PY - 2020/8

Y1 - 2020/8

N2 - Three different sources of FSH (porcine pituitary, pFSH; recombinant bovine, rbFSH; and recombinant human, rhFSH) were compared during in vitro culture of preantral and early antral follicles of goats for 18 days. Treatments were: base medium supplemented with no FSH (control), 10, 50, or 100 mIU/mL pFSH (pFSH10, pFSH50, and pFSH100, respectively), 100 ng/mL rbFSH (rbFSH), and 50 mIU/mL rhFSH (rhFSH). There were evaluations of follicle morphology, antrum formation, growth rate, estradiol production, oocyte viability and chromatin configuration, and follicle wall relative abundance of mRNA transcript for MMP-9, TIMP-2, CYP17, CYP19A1, FSHR, Insulin-R, and BAX/BCL-2 ratio. Follicle degeneration rates were similar among all treatment groups at the end of culturing. When there were treatments with pFSH, however, there was a lesser (P < 0.05) percentage of intact follicles and estradiol production, and greater (P < 0.05) extrusion rates. Furthermore, with only pFSH10 (antral follicles) and pFSH100 (preantral and antral follicles) treatments, there was a lesser (P < 0.05) follicle growth. For preantral follicles, when there was addition of pFSH10, pFSH100, and rhFSH there was lesser (P < 0.05) oocyte meiotic resumption compared to control and rbFSH treatments. For antral follicles, when there were treatments with rhFSH and pFSH10 there was greater (P = 0.08 - P < 0.05) oocyte maturation. In conclusion, the source of FSH differentially affected gene expression, as indicated by mRNA abundances, and follicular dynamics of preantral and antral follicles in vitro. Addition of FSH during the in vitro culture improved the developmental outcomes only for antral follicles.

AB - Three different sources of FSH (porcine pituitary, pFSH; recombinant bovine, rbFSH; and recombinant human, rhFSH) were compared during in vitro culture of preantral and early antral follicles of goats for 18 days. Treatments were: base medium supplemented with no FSH (control), 10, 50, or 100 mIU/mL pFSH (pFSH10, pFSH50, and pFSH100, respectively), 100 ng/mL rbFSH (rbFSH), and 50 mIU/mL rhFSH (rhFSH). There were evaluations of follicle morphology, antrum formation, growth rate, estradiol production, oocyte viability and chromatin configuration, and follicle wall relative abundance of mRNA transcript for MMP-9, TIMP-2, CYP17, CYP19A1, FSHR, Insulin-R, and BAX/BCL-2 ratio. Follicle degeneration rates were similar among all treatment groups at the end of culturing. When there were treatments with pFSH, however, there was a lesser (P < 0.05) percentage of intact follicles and estradiol production, and greater (P < 0.05) extrusion rates. Furthermore, with only pFSH10 (antral follicles) and pFSH100 (preantral and antral follicles) treatments, there was a lesser (P < 0.05) follicle growth. For preantral follicles, when there was addition of pFSH10, pFSH100, and rhFSH there was lesser (P < 0.05) oocyte meiotic resumption compared to control and rbFSH treatments. For antral follicles, when there were treatments with rhFSH and pFSH10 there was greater (P = 0.08 - P < 0.05) oocyte maturation. In conclusion, the source of FSH differentially affected gene expression, as indicated by mRNA abundances, and follicular dynamics of preantral and antral follicles in vitro. Addition of FSH during the in vitro culture improved the developmental outcomes only for antral follicles.

KW - Antral follicles

KW - FSH sources

KW - Gene expression

KW - Goat

KW - Oocyte maturation

KW - Preantral follicles

UR - http://www.scopus.com/inward/record.url?scp=85087497342&partnerID=8YFLogxK

U2 - 10.1016/j.anireprosci.2020.106461

DO - 10.1016/j.anireprosci.2020.106461

M3 - Journal article

C2 - 32828391

VL - 219

SP - 106461

JO - Animal Reproduction Science

JF - Animal Reproduction Science

SN - 0378-4320

M1 - 106461

ER -

ID: 61255567