Phage-display libraries of murine and human antibody Fab fragments

J Engberg, P S Andersen, L K Nielsen, M Dziegiel, L K Johansen, B Albrechtsen

39 Citations (Scopus)

Abstract

We provide efficient and detailed procedures for construction, expression, and screening of comprehensive libraries of murine or human antibody Fab fragments displayed on the surface of filamentous phage. In addition, protocols for producing and using ultra-electrocompetent cells, for producing Fab phages from libraries, and for selecting antigen binders by panning are presented. The latter protocol includes a procedure for trypsin elution of bound phage.

Original languageEnglish
JournalMolecular Biotechnology
Volume6
Issue number3
Pages (from-to)287-310
Number of pages24
ISSN1073-6085
DOIs
Publication statusPublished - Dec 1996

Keywords

  • Animals
  • Bacteriophages
  • Gene Library
  • Humans
  • Immunoglobulin Fab Fragments
  • Mice

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