Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test: A Prospective Community Study in Older Adults

Roy P Zuurbier; Koos Korsten; Theo J M Verheij; Chris Butler; Niels Adriaenssens; Samuel Coenen; Olivier Gruselle; Valerie Vantomme; Marlies A van Houten; Louis J Bont; Joanne G Wildenbeest; REspiratory Syncytial virus Consortium in EUrope (RESCEU) Investigators; Thea Kølsen Fischer

doi:10.1093/infdis/jiab600

Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test: A Prospective Community Study in Older Adults

Roy P Zuurbier, Koos Korsten, Theo J M Verheij, Chris Butler, Niels Adriaenssens, Samuel Coenen, Olivier Gruselle, Valerie Vantomme, Marlies A van Houten, Louis J Bont, Joanne G Wildenbeest, REspiratory Syncytial virus Consortium in EUrope (RESCEU) Investigators , Thea Kølsen Fischer (Member of author group)

Department of Clinical Research

20 Citations (Scopus)

Abstract

BACKGROUND: Respiratory syncytial virus (RSV) causes a substantial burden in older adults. Viral load in RSV-infected adults is generally lower compared to young children, which could result in suboptimal sensitivity of RSV diagnostics. Although the Xpert® Xpress Flu/RSV assay has been used in routine clinical care, its sensitivity to diagnose RSV infection in older adults is largely unknown. We aimed to compare the performance of the Xpert® Xpress Flu/RSV assay with real-time reverse-transcription polymerase chain reaction (RT-PCR) in home-dwelling older adults (≥60 years of age).

METHODS: Nasopharyngeal swabs were tested with Xpert® Xpress Flu/RSV and compared to RSV RT-PCR in older adults with acute respiratory tract infections with different levels of disease severity.

RESULTS: We studied 758 respiratory samples from 561 older adults from 2 consecutive RSV seasons. Thirty-five (4.6%) samples tested positive for RSV by at least 1 of the assays, of which 2 samples were negative by Xpert® Xpress Flu/RSV and 3 samples by real-time RT-PCR. The positive percentage agreement (PPA) was 90.9% (95% confidence interval [CI], 76.4%-96.8%) and negative percentage agreement was 99.7% (95% CI, 99.0%-99.9%). Viral loads were low (≤103 copies/mL or cycle threshold value ≥34) in all cases with discordant results for the 2 assays.

CONCLUSIONS: The PPA of Xpert® Xpress Flu/RSV compared to routine RT-PCR is high for RSV detection in home-dwelling older adults. The assay is fast and easy to use at the point of care.

CLINICAL TRIALS REGISTRATION: NCT03621930.

Original language	English
Journal	The Journal of infectious diseases
Volume	226
Issue number	Supplement_1
Pages (from-to)	S63-S70
ISSN	0022-1899
DOIs	https://doi.org/10.1093/infdis/jiab600
Publication status	Published - 12 Aug 2022

Keywords

diagnosis
molecular
older adults
point-of-care test
respiratory syncytial virus

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10.1093/infdis/jiab600

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Zuurbier, R. P., Korsten, K., Verheij, T. J. M., Butler, C., Adriaenssens, N., Coenen, S., Gruselle, O., Vantomme, V., van Houten, M. A., Bont, L. J., Wildenbeest, J. G., REspiratory Syncytial virus Consortium in EUrope (RESCEU) Investigators , & Fischer, T. K. (2022). Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test: A Prospective Community Study in Older Adults. The Journal of infectious diseases, 226(Supplement_1), S63-S70. https://doi.org/10.1093/infdis/jiab600

Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test: A Prospective Community Study in Older Adults. / Zuurbier, Roy P; Korsten, Koos; Verheij, Theo J M et al.
In: The Journal of infectious diseases, Vol. 226, No. Supplement_1, 12.08.2022, p. S63-S70.

Research output: Contribution to journal › Journal article › Research › peer-review

Zuurbier, RP, Korsten, K, Verheij, TJM, Butler, C, Adriaenssens, N, Coenen, S, Gruselle, O, Vantomme, V, van Houten, MA, Bont, LJ, Wildenbeest, JG, REspiratory Syncytial virus Consortium in EUrope (RESCEU) Investigators & Fischer, TK 2022, 'Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test: A Prospective Community Study in Older Adults', The Journal of infectious diseases, vol. 226, no. Supplement_1, pp. S63-S70. https://doi.org/10.1093/infdis/jiab600

@article{87bfada4e8884ccab4f7f1bb57e2e80f,

title = "Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test: A Prospective Community Study in Older Adults",

abstract = "BACKGROUND: Respiratory syncytial virus (RSV) causes a substantial burden in older adults. Viral load in RSV-infected adults is generally lower compared to young children, which could result in suboptimal sensitivity of RSV diagnostics. Although the Xpert{\textregistered} Xpress Flu/RSV assay has been used in routine clinical care, its sensitivity to diagnose RSV infection in older adults is largely unknown. We aimed to compare the performance of the Xpert{\textregistered} Xpress Flu/RSV assay with real-time reverse-transcription polymerase chain reaction (RT-PCR) in home-dwelling older adults (≥60 years of age).METHODS: Nasopharyngeal swabs were tested with Xpert{\textregistered} Xpress Flu/RSV and compared to RSV RT-PCR in older adults with acute respiratory tract infections with different levels of disease severity.RESULTS: We studied 758 respiratory samples from 561 older adults from 2 consecutive RSV seasons. Thirty-five (4.6\%) samples tested positive for RSV by at least 1 of the assays, of which 2 samples were negative by Xpert{\textregistered} Xpress Flu/RSV and 3 samples by real-time RT-PCR. The positive percentage agreement (PPA) was 90.9\% (95\% confidence interval [CI], 76.4\%-96.8\%) and negative percentage agreement was 99.7\% (95\% CI, 99.0\%-99.9\%). Viral loads were low (≤103 copies/mL or cycle threshold value ≥34) in all cases with discordant results for the 2 assays.CONCLUSIONS: The PPA of Xpert{\textregistered} Xpress Flu/RSV compared to routine RT-PCR is high for RSV detection in home-dwelling older adults. The assay is fast and easy to use at the point of care.CLINICAL TRIALS REGISTRATION: NCT03621930.",

keywords = "diagnosis, molecular, older adults, point-of-care test, respiratory syncytial virus",

author = "Zuurbier, \{Roy P\} and Koos Korsten and Verheij, \{Theo J M\} and Chris Butler and Niels Adriaenssens and Samuel Coenen and Olivier Gruselle and Valerie Vantomme and \{van Houten\}, \{Marlies A\} and Bont, \{Louis J\} and Wildenbeest, \{Joanne G\} and \{REspiratory Syncytial virus Consortium in EUrope (RESCEU) Investigators\} and Fischer, \{Thea K{\o}lsen\}",

note = "{\textcopyright} The Author(s) 2022. Published by Oxford University Press for the Infectious Diseases Society of America.",

year = "2022",

month = aug,

day = "12",

doi = "10.1093/infdis/jiab600",

language = "English",

volume = "226",

pages = "S63--S70",

journal = "The Journal of infectious diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "Supplement\_1",

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TY - JOUR

T1 - Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test

T2 - A Prospective Community Study in Older Adults

AU - Zuurbier, Roy P

AU - Korsten, Koos

AU - Verheij, Theo J M

AU - Butler, Chris

AU - Adriaenssens, Niels

AU - Coenen, Samuel

AU - Gruselle, Olivier

AU - Vantomme, Valerie

AU - van Houten, Marlies A

AU - Bont, Louis J

AU - Wildenbeest, Joanne G

AU - REspiratory Syncytial virus Consortium in EUrope (RESCEU) Investigators

A2 - Fischer, Thea Kølsen

PY - 2022/8/12

Y1 - 2022/8/12

N2 - BACKGROUND: Respiratory syncytial virus (RSV) causes a substantial burden in older adults. Viral load in RSV-infected adults is generally lower compared to young children, which could result in suboptimal sensitivity of RSV diagnostics. Although the Xpert® Xpress Flu/RSV assay has been used in routine clinical care, its sensitivity to diagnose RSV infection in older adults is largely unknown. We aimed to compare the performance of the Xpert® Xpress Flu/RSV assay with real-time reverse-transcription polymerase chain reaction (RT-PCR) in home-dwelling older adults (≥60 years of age).METHODS: Nasopharyngeal swabs were tested with Xpert® Xpress Flu/RSV and compared to RSV RT-PCR in older adults with acute respiratory tract infections with different levels of disease severity.RESULTS: We studied 758 respiratory samples from 561 older adults from 2 consecutive RSV seasons. Thirty-five (4.6%) samples tested positive for RSV by at least 1 of the assays, of which 2 samples were negative by Xpert® Xpress Flu/RSV and 3 samples by real-time RT-PCR. The positive percentage agreement (PPA) was 90.9% (95% confidence interval [CI], 76.4%-96.8%) and negative percentage agreement was 99.7% (95% CI, 99.0%-99.9%). Viral loads were low (≤103 copies/mL or cycle threshold value ≥34) in all cases with discordant results for the 2 assays.CONCLUSIONS: The PPA of Xpert® Xpress Flu/RSV compared to routine RT-PCR is high for RSV detection in home-dwelling older adults. The assay is fast and easy to use at the point of care.CLINICAL TRIALS REGISTRATION: NCT03621930.

AB - BACKGROUND: Respiratory syncytial virus (RSV) causes a substantial burden in older adults. Viral load in RSV-infected adults is generally lower compared to young children, which could result in suboptimal sensitivity of RSV diagnostics. Although the Xpert® Xpress Flu/RSV assay has been used in routine clinical care, its sensitivity to diagnose RSV infection in older adults is largely unknown. We aimed to compare the performance of the Xpert® Xpress Flu/RSV assay with real-time reverse-transcription polymerase chain reaction (RT-PCR) in home-dwelling older adults (≥60 years of age).METHODS: Nasopharyngeal swabs were tested with Xpert® Xpress Flu/RSV and compared to RSV RT-PCR in older adults with acute respiratory tract infections with different levels of disease severity.RESULTS: We studied 758 respiratory samples from 561 older adults from 2 consecutive RSV seasons. Thirty-five (4.6%) samples tested positive for RSV by at least 1 of the assays, of which 2 samples were negative by Xpert® Xpress Flu/RSV and 3 samples by real-time RT-PCR. The positive percentage agreement (PPA) was 90.9% (95% confidence interval [CI], 76.4%-96.8%) and negative percentage agreement was 99.7% (95% CI, 99.0%-99.9%). Viral loads were low (≤103 copies/mL or cycle threshold value ≥34) in all cases with discordant results for the 2 assays.CONCLUSIONS: The PPA of Xpert® Xpress Flu/RSV compared to routine RT-PCR is high for RSV detection in home-dwelling older adults. The assay is fast and easy to use at the point of care.CLINICAL TRIALS REGISTRATION: NCT03621930.

KW - diagnosis

KW - molecular

KW - older adults

KW - point-of-care test

KW - respiratory syncytial virus

UR - https://www.scopus.com/pages/publications/85136341842

U2 - 10.1093/infdis/jiab600

DO - 10.1093/infdis/jiab600

M3 - Journal article

C2 - 35134954

SN - 0022-1899

VL - 226

SP - S63-S70

JO - The Journal of infectious diseases

JF - The Journal of infectious diseases

IS - Supplement_1

ER -

Performance Assessment of a Rapid Molecular Respiratory Syncytial Virus Point-of-Care Test: A Prospective Community Study in Older Adults

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Keywords

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