PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene; the genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes

L J Nellemann; J Frederiksen; N Morling

doi:10.1177/135245859500100310

PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene; the genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes

L J Nellemann, J Frederiksen, N Morling

8 Citations (Scopus)

Abstract

We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.

Original language	English
Journal	Multiple Sclerosis Journal
Volume	1
Issue number	3
Pages (from-to)	186-9
Number of pages	4
ISSN	1352-4585
DOIs	https://doi.org/10.1177/135245859500100310
Publication status	Published - Nov 1995
Externally published	Yes

Keywords

Alleles
Chromosome Deletion
Cloning, Molecular
DNA/analysis
Denmark/epidemiology
Gene Frequency
Genes
Genetic Predisposition to Disease
Humans
Microsatellite Repeats
Multiple Sclerosis/epidemiology
Myelin Basic Protein/classification
Optic Neuritis/epidemiology
Polymerase Chain Reaction

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PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene; the genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes. / Nellemann, L J; Frederiksen, J; Morling, N.
In: Multiple Sclerosis Journal, Vol. 1, No. 3, 11.1995, p. 186-9.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene; the genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes",

abstract = "We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.",

keywords = "Alleles, Chromosome Deletion, Cloning, Molecular, DNA/analysis, Denmark/epidemiology, Gene Frequency, Genes, Genetic Predisposition to Disease, Humans, Microsatellite Repeats, Multiple Sclerosis/epidemiology, Myelin Basic Protein/classification, Optic Neuritis/epidemiology, Polymerase Chain Reaction",

author = "Nellemann, \{L J\} and J Frederiksen and N Morling",

year = "1995",

month = nov,

doi = "10.1177/135245859500100310",

language = "English",

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pages = "186--9",

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T1 - PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene; the genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes

AU - Nellemann, L J

AU - Frederiksen, J

AU - Morling, N

PY - 1995/11

Y1 - 1995/11

N2 - We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.

AB - We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.

KW - Alleles

KW - Chromosome Deletion

KW - Cloning, Molecular

KW - DNA/analysis

KW - Denmark/epidemiology

KW - Gene Frequency

KW - Genes

KW - Genetic Predisposition to Disease

KW - Humans

KW - Microsatellite Repeats

KW - Multiple Sclerosis/epidemiology

KW - Myelin Basic Protein/classification

KW - Optic Neuritis/epidemiology

KW - Polymerase Chain Reaction

UR - https://www.scopus.com/pages/publications/0029395702

U2 - 10.1177/135245859500100310

DO - 10.1177/135245859500100310

M3 - Journal article

C2 - 9345452

SN - 1352-4585

VL - 1

SP - 186

EP - 189

JO - Multiple Sclerosis Journal

JF - Multiple Sclerosis Journal

IS - 3

ER -

PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene; the genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes

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Keywords

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