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Parallel plate model for trabecular bone exhibits volume fraction-dependent bias

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Day, J S ; Ding, M ; Odgaard, A et al. / Parallel plate model for trabecular bone exhibits volume fraction-dependent bias. In: Bone. 2000 ; Vol. 27, No. 5. pp. 715-20.

Bibtex

@article{3bd71f199aa04372b6e55bb6880651f7,
title = "Parallel plate model for trabecular bone exhibits volume fraction-dependent bias",
abstract = "Unbiased stereological methods were used in conjunction with microcomputed tomographic (micro-CT) scans of human and animal bone to investigate errors created when the parallel plate model was used to calculate morphometric parameters. Bone samples were obtained from the human proximal tibia, canine distal femur, rat tail, and pig spine and scanned in a micro-CT scanner. Trabecular thickness, trabecular spacing, and trabecular number were calculated using the parallel plate model. Direct thickness, and spacing and connectivity density were calculated using unbiased three-dimensional methods. Both thickness and spacing calculated using the plate model were well correlated to the direct three-dimensional measures (r(2) = 0. 77-0.92). The correlation between trabecular number and connectivity density varied greatly (r(2) = 0.41-0.94). Whereas trabecular thickness was consistently underestimated using the plate model, trabecular spacing was underestimated at low volume fractions and overestimated at high volume fractions. Use of the plate model resulted in a volume-dependent bias in measures of thickness and spacing (p < 0.001). This was a result of the fact that samples of low volume fraction were much more {"}rod-like{"} than those of the higher volume fraction. Our findings indicate that the plate model provides biased results, especially when populations with different volume fractions are compared. Therefore, we recommend direct thickness measures when three-dimensional data sets are available.",
keywords = "Adolescent, Adult, Aged, Aged, 80 and over, Animals, Bone and Bones/anatomy & histology, Dogs, Humans, Middle Aged, Rats, Swine, Tomography, X-Ray Computed",
author = "Day, {J S} and M Ding and A Odgaard and Sumner, {D R} and I Hvid and H Weinans",
year = "2000",
month = nov,
doi = "10.1016/s8756-3282(00)00371-9",
language = "English",
volume = "27",
pages = "715--20",
journal = "Bone",
issn = "1873-2763",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Parallel plate model for trabecular bone exhibits volume fraction-dependent bias

AU - Day, J S

AU - Ding, M

AU - Odgaard, A

AU - Sumner, D R

AU - Hvid, I

AU - Weinans, H

PY - 2000/11

Y1 - 2000/11

N2 - Unbiased stereological methods were used in conjunction with microcomputed tomographic (micro-CT) scans of human and animal bone to investigate errors created when the parallel plate model was used to calculate morphometric parameters. Bone samples were obtained from the human proximal tibia, canine distal femur, rat tail, and pig spine and scanned in a micro-CT scanner. Trabecular thickness, trabecular spacing, and trabecular number were calculated using the parallel plate model. Direct thickness, and spacing and connectivity density were calculated using unbiased three-dimensional methods. Both thickness and spacing calculated using the plate model were well correlated to the direct three-dimensional measures (r(2) = 0. 77-0.92). The correlation between trabecular number and connectivity density varied greatly (r(2) = 0.41-0.94). Whereas trabecular thickness was consistently underestimated using the plate model, trabecular spacing was underestimated at low volume fractions and overestimated at high volume fractions. Use of the plate model resulted in a volume-dependent bias in measures of thickness and spacing (p < 0.001). This was a result of the fact that samples of low volume fraction were much more "rod-like" than those of the higher volume fraction. Our findings indicate that the plate model provides biased results, especially when populations with different volume fractions are compared. Therefore, we recommend direct thickness measures when three-dimensional data sets are available.

AB - Unbiased stereological methods were used in conjunction with microcomputed tomographic (micro-CT) scans of human and animal bone to investigate errors created when the parallel plate model was used to calculate morphometric parameters. Bone samples were obtained from the human proximal tibia, canine distal femur, rat tail, and pig spine and scanned in a micro-CT scanner. Trabecular thickness, trabecular spacing, and trabecular number were calculated using the parallel plate model. Direct thickness, and spacing and connectivity density were calculated using unbiased three-dimensional methods. Both thickness and spacing calculated using the plate model were well correlated to the direct three-dimensional measures (r(2) = 0. 77-0.92). The correlation between trabecular number and connectivity density varied greatly (r(2) = 0.41-0.94). Whereas trabecular thickness was consistently underestimated using the plate model, trabecular spacing was underestimated at low volume fractions and overestimated at high volume fractions. Use of the plate model resulted in a volume-dependent bias in measures of thickness and spacing (p < 0.001). This was a result of the fact that samples of low volume fraction were much more "rod-like" than those of the higher volume fraction. Our findings indicate that the plate model provides biased results, especially when populations with different volume fractions are compared. Therefore, we recommend direct thickness measures when three-dimensional data sets are available.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Bone and Bones/anatomy & histology

KW - Dogs

KW - Humans

KW - Middle Aged

KW - Rats

KW - Swine

KW - Tomography, X-Ray Computed

U2 - 10.1016/s8756-3282(00)00371-9

DO - 10.1016/s8756-3282(00)00371-9

M3 - Journal article

C2 - 11062361

VL - 27

SP - 715

EP - 720

JO - Bone

JF - Bone

SN - 1873-2763

IS - 5

ER -

ID: 78347883