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Oral insulin does not alter gut microbiota composition of NOD mice

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Kihl, P, Krych, L, Buschard, K, Wesley, JD, Kot, W, Hansen, AK, Nielsen, DS & von Herrath, MG 2018, 'Oral insulin does not alter gut microbiota composition of NOD mice', Diabetes - Metabolism: Research and Reviews (Print Edition), vol. 34, no. 6, pp. e3010. https://doi.org/10.1002/dmrr.3010

APA

Kihl, P., Krych, L., Buschard, K., Wesley, J. D., Kot, W., Hansen, A. K., Nielsen, D. S., & von Herrath, M. G. (2018). Oral insulin does not alter gut microbiota composition of NOD mice. Diabetes - Metabolism: Research and Reviews (Print Edition), 34(6), e3010. https://doi.org/10.1002/dmrr.3010

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MLA

Vancouver

Author

Kihl, Pernille ; Krych, Lukasz ; Buschard, Karsten ; Wesley, Johnna D ; Kot, Witold ; Hansen, Axel Kornerup ; Nielsen, Dennis S ; von Herrath, Matthias G. / Oral insulin does not alter gut microbiota composition of NOD mice. In: Diabetes - Metabolism: Research and Reviews (Print Edition). 2018 ; Vol. 34, No. 6. pp. e3010.

Bibtex

@article{6362eed90ebf4eebaf50e3fd9521c9f0,
title = "Oral insulin does not alter gut microbiota composition of NOD mice",
abstract = "BACKGROUND: Oral insulin as a preventive strategy and/or treatment of type 1 diabetes has been the target of much research. Producing oral insulins is a complex and challenging task, with numerous pitfalls, due to physiological, physical, and biochemical barriers. Our aim was to determine the impact of oral insulin on the delicate gut microbiota composition.METHODS: Female nonobese diabetic mice were given oral porcine insulin 2 times a week from 5 weeks of age for 4 weeks, and then subsequently once a week for 21 weeks, or until euthanized. The mice were divided into groups on a gluten-reduced diet or a standard diet. Gut microbiota composition was analysed based on faecal samples, and the type 1 diabetes incidence of the mice was monitored.RESULTS: We observed no influence of the oral porcine insulin on the gut microbiota composition of mice on a gluten-reduced or a standard diet at 9 weeks of age. Also, the administration of oral insulin did not influence the incidence of type 1 diabetes at 30 weeks of age.CONCLUSIONS: Oral porcine insulin does not alter the gut microbiota composition of nonobese diabetic mice on either a gluten-reduced diet or standard diet. Also, the oral porcine insulin did not influence the incidence of type 1 diabetes in the groups.",
keywords = "Administration, Oral, Animals, Diabetes Mellitus, Experimental/drug therapy, Diabetes Mellitus, Type 1/drug therapy, Dysbiosis/immunology, Feces/microbiology, Female, Gastrointestinal Microbiome/drug effects, Insulin, Regular, Pork/administration & dosage, Mice, Mice, Inbred NOD, Swine",
author = "Pernille Kihl and Lukasz Krych and Karsten Buschard and Wesley, {Johnna D} and Witold Kot and Hansen, {Axel Kornerup} and Nielsen, {Dennis S} and {von Herrath}, {Matthias G}",
note = "Copyright {\textcopyright} 2018 John Wiley & Sons, Ltd.",
year = "2018",
month = sep,
doi = "10.1002/dmrr.3010",
language = "English",
volume = "34",
pages = "e3010",
journal = "Diabetes - Metabolism: Research and Reviews",
issn = "1520-7552",
publisher = "John/Wiley & Sons Ltd",
number = "6",

}

RIS

TY - JOUR

T1 - Oral insulin does not alter gut microbiota composition of NOD mice

AU - Kihl, Pernille

AU - Krych, Lukasz

AU - Buschard, Karsten

AU - Wesley, Johnna D

AU - Kot, Witold

AU - Hansen, Axel Kornerup

AU - Nielsen, Dennis S

AU - von Herrath, Matthias G

N1 - Copyright © 2018 John Wiley & Sons, Ltd.

PY - 2018/9

Y1 - 2018/9

N2 - BACKGROUND: Oral insulin as a preventive strategy and/or treatment of type 1 diabetes has been the target of much research. Producing oral insulins is a complex and challenging task, with numerous pitfalls, due to physiological, physical, and biochemical barriers. Our aim was to determine the impact of oral insulin on the delicate gut microbiota composition.METHODS: Female nonobese diabetic mice were given oral porcine insulin 2 times a week from 5 weeks of age for 4 weeks, and then subsequently once a week for 21 weeks, or until euthanized. The mice were divided into groups on a gluten-reduced diet or a standard diet. Gut microbiota composition was analysed based on faecal samples, and the type 1 diabetes incidence of the mice was monitored.RESULTS: We observed no influence of the oral porcine insulin on the gut microbiota composition of mice on a gluten-reduced or a standard diet at 9 weeks of age. Also, the administration of oral insulin did not influence the incidence of type 1 diabetes at 30 weeks of age.CONCLUSIONS: Oral porcine insulin does not alter the gut microbiota composition of nonobese diabetic mice on either a gluten-reduced diet or standard diet. Also, the oral porcine insulin did not influence the incidence of type 1 diabetes in the groups.

AB - BACKGROUND: Oral insulin as a preventive strategy and/or treatment of type 1 diabetes has been the target of much research. Producing oral insulins is a complex and challenging task, with numerous pitfalls, due to physiological, physical, and biochemical barriers. Our aim was to determine the impact of oral insulin on the delicate gut microbiota composition.METHODS: Female nonobese diabetic mice were given oral porcine insulin 2 times a week from 5 weeks of age for 4 weeks, and then subsequently once a week for 21 weeks, or until euthanized. The mice were divided into groups on a gluten-reduced diet or a standard diet. Gut microbiota composition was analysed based on faecal samples, and the type 1 diabetes incidence of the mice was monitored.RESULTS: We observed no influence of the oral porcine insulin on the gut microbiota composition of mice on a gluten-reduced or a standard diet at 9 weeks of age. Also, the administration of oral insulin did not influence the incidence of type 1 diabetes at 30 weeks of age.CONCLUSIONS: Oral porcine insulin does not alter the gut microbiota composition of nonobese diabetic mice on either a gluten-reduced diet or standard diet. Also, the oral porcine insulin did not influence the incidence of type 1 diabetes in the groups.

KW - Administration, Oral

KW - Animals

KW - Diabetes Mellitus, Experimental/drug therapy

KW - Diabetes Mellitus, Type 1/drug therapy

KW - Dysbiosis/immunology

KW - Feces/microbiology

KW - Female

KW - Gastrointestinal Microbiome/drug effects

KW - Insulin, Regular, Pork/administration & dosage

KW - Mice

KW - Mice, Inbred NOD

KW - Swine

U2 - 10.1002/dmrr.3010

DO - 10.1002/dmrr.3010

M3 - Journal article

C2 - 29637693

VL - 34

SP - e3010

JO - Diabetes - Metabolism: Research and Reviews

JF - Diabetes - Metabolism: Research and Reviews

SN - 1520-7552

IS - 6

ER -

ID: 56440883