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Oral insulin does not alter gut microbiota composition of NOD mice

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  • Pernille Kihl
  • Lukasz Krych
  • Karsten Buschard
  • Johnna D Wesley
  • Witold Kot
  • Axel Kornerup Hansen
  • Dennis S Nielsen
  • Matthias G von Herrath
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BACKGROUND: Oral insulin as a preventive strategy and/or treatment of type 1 diabetes has been the target of much research. Producing oral insulins is a complex and challenging task, with numerous pitfalls, due to physiological, physical, and biochemical barriers. Our aim was to determine the impact of oral insulin on the delicate gut microbiota composition.

METHODS: Female nonobese diabetic mice were given oral porcine insulin 2 times a week from 5 weeks of age for 4 weeks, and then subsequently once a week for 21 weeks, or until euthanized. The mice were divided into groups on a gluten-reduced diet or a standard diet. Gut microbiota composition was analysed based on faecal samples, and the type 1 diabetes incidence of the mice was monitored.

RESULTS: We observed no influence of the oral porcine insulin on the gut microbiota composition of mice on a gluten-reduced or a standard diet at 9 weeks of age. Also, the administration of oral insulin did not influence the incidence of type 1 diabetes at 30 weeks of age.

CONCLUSIONS: Oral porcine insulin does not alter the gut microbiota composition of nonobese diabetic mice on either a gluten-reduced diet or standard diet. Also, the oral porcine insulin did not influence the incidence of type 1 diabetes in the groups.

Original languageEnglish
JournalDiabetes - Metabolism: Research and Reviews (Print Edition)
Volume34
Issue number6
Pages (from-to)e3010
ISSN1520-7552
DOIs
Publication statusPublished - Sep 2018

    Research areas

  • Administration, Oral, Animals, Diabetes Mellitus, Experimental/drug therapy, Diabetes Mellitus, Type 1/drug therapy, Dysbiosis/immunology, Feces/microbiology, Female, Gastrointestinal Microbiome/drug effects, Insulin, Regular, Pork/administration & dosage, Mice, Mice, Inbred NOD, Swine

ID: 56440883