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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells

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DOI

  1. Correlation of MET-Receptor Overexpression with MET Gene Amplification and Patient Outcome in Malignant Mesothelioma

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  2. The collagen receptor uparap in malignant mesothelioma: A potential diagnostic marker and therapeutic target

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  1. Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity

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  2. A simple method for detecting oncofetal chondroitin sulfate glycosaminoglycans in bladder cancer urine

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  3. Dynamics of PfEMP1 Antibody Profile From Birth to 12 Months of Age in Beninese Infants

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Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.

Original languageEnglish
JournalInternational Journal of Molecular Sciences
Volume21
Issue number7
ISSN1661-6596
DOIs
Publication statusPublished - 31 Mar 2020

    Research areas

  • Antigens, Protozoan/genetics, Cell Line, Tumor, Chondroitin Sulfates/metabolism, Early Detection of Cancer/methods, Humans, Magnetics, Neoplastic Cells, Circulating/pathology, Recombinant Proteins

ID: 62343700