Ocular proteomics with emphasis on two-dimensional gel electrophoresis and mass spectrometry

Nakul Mandal, Steffen Heegaard, Jan Ulrik Prause, Bent Honoré, Henrik Vorum

27 Citations (Scopus)

Abstract

The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Appropriate sample preparation for the diverse range of cells and tissues of the eye is essential to ensure reliable results. Current methods of protein staining for 2D-PAGE, protein labelling for two-dimensional difference gel electrophoresis, gel-based expression analysis and protein identification by MS are summarised. The uses of gel-free MS-based strategies (MuDPIT, iTRAQ, ICAT and SILAC) are also discussed. Proteomic technologies promise to shed new light onto ocular disease processes that could lead to the discovery of strong novel biomarkers and therapeutic targets useful in many ophthalmic conditions.

Original languageEnglish
JournalBiological Procedures Online
Volume12
Issue number1
Pages (from-to)56-88
Number of pages33
ISSN1480-9222
DOIs
Publication statusPublished - 24 Dec 2009
Externally publishedYes

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