Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

Mario Corral-Bolaños; Tram Nguyen; Hans Martin Kjer; Marco Pizzolato; Casper Bo Gravesen Salinas; Johanna Perens; Jeppe Revall Frisvad; Julian  Colombelli; Tim B Dyrby

Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

Mario Corral-Bolaños, Tram Nguyen, Hans Martin Kjer, Marco Pizzolato, Casper Bo Gravesen Salinas, Johanna Perens, Jeppe Revall Frisvad, Julian Colombelli, Tim B Dyrby

Abstract

This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.

Original language	English
Publication date	Jun 2023
Number of pages	2
Publication status	Published - Jun 2023
Event	2023 ISMRM & ISMRT Annual Meeting & Exhibition - Metro Toronto Convention Centre, Toronto, Canada Duration: 3 Jun 2023 → 8 Jun 2023 https://www.ismrm.org/23m/

Conference

Conference	2023 ISMRM & ISMRT Annual Meeting & Exhibition
Location	Metro Toronto Convention Centre
Country/Territory	Canada
City	Toronto
Period	03/06/2023 → 08/06/2023
Internet address	https://www.ismrm.org/23m/

Cite this

Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter. / Corral-Bolaños, Mario; Nguyen, Tram; Kjer, Hans Martin et al.
2023. 1-2 Abstract from 2023 ISMRM & ISMRT Annual Meeting & Exhibition, Toronto, Ontario, Canada.

Research output: Contribution to conference › Conference abstract for conference › Research

@conference{44fc748bd8fc42b6acc0c840b2ff03f4,

title = "Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter",

abstract = "This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.",

author = "Mario Corral-Bola{\~n}os and Tram Nguyen and Kjer, {Hans Martin} and Marco Pizzolato and Salinas, {Casper Bo Gravesen} and Johanna Perens and Frisvad, {Jeppe Revall} and Julian Colombelli and Dyrby, {Tim B}",

year = "2023",

month = jun,

language = "English",

pages = "1--2",

note = "2023 ISMRM & ISMRT Annual Meeting & Exhibition, ISMRM ; Conference date: 03-06-2023 Through 08-06-2023",

url = "https://www.ismrm.org/23m/",

}

TY - ABST

T1 - Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

AU - Corral-Bolaños, Mario

AU - Nguyen, Tram

AU - Kjer, Hans Martin

AU - Pizzolato, Marco

AU - Salinas, Casper Bo Gravesen

AU - Perens, Johanna

AU - Frisvad, Jeppe Revall

AU - Colombelli, Julian

AU - Dyrby, Tim B

PY - 2023/6

Y1 - 2023/6

N2 - This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.

AB - This work develops a novel 3D validation technique that reveals the white matter orientation with micron resolution in cleared murine brains. Light-sheet elastic scattering microscopy (LSSM) can be used to exploit the scattering signal exhibited by white matter fibres, which is dependent on their orientation. The rotation and imaging of the sample yield a scattering profile, which corresponds to that of infinitely long cylinders. Our work combines two orthogonal acquisitions to voxel-wise reconstruct 3D-fibre orientations in large brain volumes. LSSM could be the definitive validation method for diffusion MRI orientation methods.

M3 - Conference abstract for conference

SP - 1

EP - 2

T2 - 2023 ISMRM & ISMRT Annual Meeting & Exhibition

Y2 - 3 June 2023 through 8 June 2023

ER -

Novel light-sheet scattering microscopy for voxel-wise validation of 3D-fibre orientations in murine white matter

Abstract

Conference

Fingerprint

Cite this