Northern blotting analysis

Knud Josefsen, Henrik Nielsen

25 Citations (Scopus)

Abstract

Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.
Original languageEnglish
JournalMethods in Molecular Biology
Volume703
Pages (from-to)87-105
Number of pages19
ISSN1064-3745
DOIs
Publication statusPublished - 2011

Keywords

  • Blotting, Northern
  • Electrophoresis, Agar Gel
  • Electrophoresis, Polyacrylamide Gel
  • Formaldehyde
  • Glyoxal
  • Membranes, Artificial
  • RNA

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