Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Non-image Forming Light Detection by Melanopsin, Rhodopsin, and Long-Middlewave (L/W) Cone Opsin in the Subterranean Blind Mole Rat, Spalax Ehrenbergi: Immunohistochemical Characterization, Distribution, and Connectivity

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Editorial: Neurostereology

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Using the Optical Fractionator to Estimate Total Cell Numbers in the Normal and Abnormal Developing Human Forebrain

    Research output: Contribution to journalReviewResearchpeer-review

  3. Central melanopsin projections in the diurnal rodent, Arvicanthis niloticus

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Prevalence of increases in functional connectivity in visual, somatosensory and language areas in congenital blindness

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Quantitative relationships in delphinid neocortex

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Spatiotemporal expression pattern of PERIOD 1 and PERIOD 2 in the mouse SCN is dependent on VIP receptor 2 signaling

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Mice Lacking EGR1 Have Impaired Clock Gene (BMAL1) Oscillation, Locomotor Activity, and Body Temperature

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Døgnrytmer, søvn og søvnforstyrrelser

    Research output: Chapter in Book/Report/Conference proceedingBook chapterEducationpeer-review

  4. Localization, distribution, and connectivity of neuropeptide Y in the human and porcine retinas - a comparative study

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

The blind mole rat, Spalax ehrenbergi, can, despite severely degenerated eyes covered by fur, entrain to the daily light/dark cycle and adapt to seasonal changes due to an intact circadian timing system. The present study demonstrates that the Spalax retina contains a photoreceptor layer, an outer nuclear layer (ONL), an outer plexiform layer (OPL), an inner nuclear layer (INL), an inner plexiform layer (IPL), and a ganglion cell layer (GCL). By immunohistochemistry, the number of melanopsin (mRGCs) and non-melanopsin bearing retinal ganglion cells was analyzed in detail. Using the ganglion cell marker RNA-binding protein with multiple splicing (RBPMS) it was shown that the Spalax eye contains 890 ± 62 RGCs. Of these, 87% (752 ± 40) contain melanopsin (cell density 788 melanopsin RGCs/mm(2)). The remaining RGCs were shown to co-store Brn3a and calretinin. The melanopsin cells were located mainly in the GCL with projections forming two dendritic plexuses located in the inner part of the IPL and in the OPL. Few melanopsin dendrites were also found in the ONL. The Spalax retina is rich in rhodopsin and long/middle wave (L/M) cone opsin bearing photoreceptor cells. By using Ctbp2 as a marker for ribbon synapses, both rods and L/M cone ribbons containing pedicles in the OPL were found in close apposition with melanopsin dendrites in the outer plexus suggesting direct synaptic contact. A subset of cone bipolar cells and all photoreceptor cells contain recoverin while a subset of bipolar and amacrine cells contain calretinin. The calretinin expressing amacrine cells seemed to form synaptic contacts with rhodopsin containing photoreceptor cells in the OPL and contacts with melanopsin cell bodies and dendrites in the IPL. The study demonstrates the complex retinal circuitry used by the Spalax to detect light, and provides evidence for both melanopsin and non-melanopsin projecting pathways to the brain.

Original languageEnglish
JournalFrontiers in Neuroanatomy
Volume10
Pages (from-to)61
ISSN1662-5129
DOIs
Publication statusPublished - 9 Jun 2016

    Research areas

  • Journal Article

ID: 49846293