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New Nordic Diet-Induced Weight Loss Is Accompanied by Changes in Metabolism and AMPK Signaling in Adipose Tissue

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  • Andreas Mæchel Fritzen
  • Anne-Marie Lundsgaard
  • Andreas Børsting Jordy
  • Sanne Kellebjerg Poulsen
  • Steen Stender
  • Henriette Pilegaard
  • Arne Astrup
  • Thomas Meinert Larsen
  • Jørgen F P Wojtaszewski
  • Erik A Richter
  • Bente Kiens
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CONTEXT: The molecular mechanisms behind diet-induced metabolic improvements remain to be studied.

OBJECTIVE: This study sought to investigate whether expression of proteins in skeletal muscle or adipose tissue could explain improvements in glucose and lipid homeostasis after weight loss.

DESIGN: Volunteers consumed a New Nordic Diet (NND) or an Average Danish Diet for 26 weeks in a controlled, free-living setting.

SUBJECTS: Sixty four moderately obese women and men (44 ± 2 y; body mass index, 31 ± 1 kg/m(2)).

INTERVENTION: Fasting blood samples and biopsies from the vastus lateralis muscle and subcutaneous abdominal adipose tissue (SCAT) were obtained at week 0 and 26.

OUTCOME: Gene and protein expressions were analyzed by real-time PCR and Western blotting.

RESULTS: Improved homeostasis homeostatic model of assessment-insulin resistance index and lowered plasma triacylglycerol concentration after NND coincided with molecular adaptations in SCAT but not in skeletal muscle. NND induced greater reduction in fat mass than ADD (-6 ± 1 kg and -2 ± 1 kg; P < .01). In SCAT this was associated with increased AMPK and acetyl-CoA carboxylase phosphorylation (P < .05). Concomitantly, NND induced up-regulation of Akt2 and Akt substrate of 160 kDa (P < .05) as well as fatty acid transport protein 4 and membrane associated fatty acid binding protein (P < .05). Indices of increased oxidative capacity were observed, as carnitine palmitoyl transferase 1 mRNA (P = .08) as well as citrate synthase (P = .1) and cytochrome c (P = .05) protein tended to increase.

CONCLUSION: NND-induced metabolic improvements were accompanied by increased AMPK signaling in SCAT, suggesting a role of AMPK in these adaptations. The concomitant up-regulation of key glucose and lipid-handling proteins suggests an improved metabolic capacity in adipose tissue after weight loss.

Original languageEnglish
JournalThe Journal of clinical endocrinology and metabolism
Volume100
Issue number9
Pages (from-to)3509-19
Number of pages11
ISSN0021-972X
DOIs
Publication statusPublished - Sep 2015

    Research areas

  • AMP-Activated Protein Kinases, Adipose Tissue, Adult, Blood Glucose, Body Mass Index, Diet, Reducing, Energy Metabolism, Female, Humans, Insulin Resistance, Male, Middle Aged, Muscle, Skeletal, Obesity, Signal Transduction, Weight Loss

ID: 45952421