Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Neurotrophic Effects of Vascular Endothelial Growth Factor B and Novel Mimetic Peptides on Neurons from the Central Nervous System

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Development and Evaluation of Two Potential 5-HT7 Receptor PET Tracers: [18F]ENL09 and [18F]ENL10

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Development and Evaluation of Two Potential 5-HT7Receptor PET Tracers: [18F]ENL09 and [18F]ENL10

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Classics in Neuroimaging: The Serotonergic 2A Receptor System-from Discovery to Modern Molecular Imaging

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Short erythropoietin-derived peptide enhances memory, improves long-term potentiation, and counteracts amyloid beta-induced pathology

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Gut and immune effects of bioactive milk factors in preterm pigs exposed to prenatal inflammation

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Bovine Milk Oligosaccharides with Sialyllactose Improves Cognition in Preterm Pigs

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Artemin and an Artemin-Derived Peptide, Artefin, Induce Neuronal Survival, and Differentiation Through Ret and NCAM

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Erythropoietin-derived peptide NL100 improves memory in healthy rats and in a rat model of Alzheimer's disease

    Research output: Contribution to conferencePosterResearchpeer-review

  • Oksana Dmytriyeva
  • Amaia de Diego Ajenjo
  • Kathrine Lundø
  • Henrik Hertz
  • Kim K Rasmussen
  • Anders T Christiansen
  • Jorg Klingelhofer
  • Alexander L Nielsen
  • Jan Hoeber
  • Elena Kozlova
  • David P D Woldbye
  • Stanislava Pankratova
View graph of relations

Vascular endothelial growth factor B (VEGFB) is a pleiotropic trophic factor, which in contrast to the closely related VEGFA is known to have a limited effect on angiogenesis. VEGFB improves survival in various tissues including the nervous system, where the effect was observed mainly for peripheral neurons. The neurotrophic effect of VEGFB on central nervous system neurons has been less investigated. Here we demonstrated that VEGFB promotes neurite outgrowth from primary cerebellar granule, hippocampal, and retinal neurons in vitro. VEGFB protected hippocampal and retinal neurons from both oxidative stress and glutamate-induced neuronal death. The VEGF receptor 1 (VEGFR1) is required for VEGFB-induced neurotrophic and neuroprotective effects. Using a structure-based approach, we designed short peptides, termed Vefin1-7, mimicking the binding interface of VEGFB to VEGFR1. Vefins were analyzed for their secondary structure and binding to VEGF receptors and compared with previously described peptides derived from VEGFA, another ligand of VEGFR1. We show that Vefins have neurotrophic and neuroprotective effects on primary hippocampal, cerebellar granule, and retinal neurons in vitro with potencies comparable to VEGFB. Similar to VEGFB, Vefins were not mitogenic for MCF-7 cancer cells. Furthermore, one of the peptides, Vefin7, even dose-dependently inhibited the proliferation of MCF-7 cells in vitro. Unraveling the neurotrophic and neuroprotective potentials of VEGFB, the only nonangiogenic factor of the VEGF family, is promising for the development of neuroprotective peptide-based therapies.

Original languageEnglish
JournalACS Chemical Neuroscience
Volume11
Issue number9
Pages (from-to)1270-1282
Number of pages13
ISSN1948-7193
DOIs
Publication statusPublished - 6 May 2020

ID: 64730421