Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series

Karin Holst Lauridsen; Kristine Boisen Olsen; Eva Løbner Lund; Tomas O Jensen; Thomas Ingemann Pedersen; Zitta Barrella Harboe; Valeria Antsupova; Lasse Dam Rasmussen; Dennis Röser; Jytte Banner; Kristina Træholt Franck; Veronika Vorobieva Solholm Jensen

doi:10.1177/2632010X221139096

Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series

Karin Holst Lauridsen^*, Kristine Boisen Olsen, Eva Løbner Lund, Tomas O Jensen, Thomas Ingemann Pedersen, Zitta Barrella Harboe, Valeria Antsupova, Lasse Dam Rasmussen, Dennis Röser, Jytte Banner, Kristina Træholt Franck, Veronika Vorobieva Solholm Jensen

^*Corresponding author for this work

2 Citations (Scopus)

Abstract

BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.

METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.

CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.

Original language	English
Journal	Clinical pathology (Thousand Oaks, Ventura County, Calif.)
Volume	15
Pages (from-to)	2632010X221139096
ISSN	2632-010X
DOIs	https://doi.org/10.1177/2632010X221139096
Publication status	Published - Dec 2022

Keywords

Antibodies
SARS virus
autopsy
case reports
coronavirus
immunohistochemistry

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Lauridsen, K. H., Olsen, K. B., Lund, E. L., Jensen, T. O., Pedersen, T. I., Harboe, Z. B., Antsupova, V., Rasmussen, L. D., Röser, D., Banner, J., Franck, K. T., & Vorobieva Solholm Jensen, V. (2022). Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. Clinical pathology (Thousand Oaks, Ventura County, Calif.), 15, 2632010X221139096. https://doi.org/10.1177/2632010X221139096

Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. / Lauridsen, Karin Holst; Olsen, Kristine Boisen; Lund, Eva Løbner et al.
In: Clinical pathology (Thousand Oaks, Ventura County, Calif.), Vol. 15, 12.2022, p. 2632010X221139096.

Research output: Contribution to journal › Journal article › Research › peer-review

Lauridsen, KH, Olsen, KB, Lund, EL, Jensen, TO, Pedersen, TI , Harboe, ZB , Antsupova, V, Rasmussen, LD, Röser, D, Banner, J, Franck, KT & Vorobieva Solholm Jensen, V 2022, 'Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series', Clinical pathology (Thousand Oaks, Ventura County, Calif.), vol. 15, pp. 2632010X221139096. https://doi.org/10.1177/2632010X221139096

Lauridsen KH, Olsen KB, Lund EL, Jensen TO, Pedersen TI , Harboe ZB et al. Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. Clinical pathology (Thousand Oaks, Ventura County, Calif.). 2022 Dec;15:2632010X221139096. doi: 10.1177/2632010X221139096

Lauridsen, Karin Holst ; Olsen, Kristine Boisen ; Lund, Eva Løbner et al. / Neurological Complications in COVID-19 Patients : Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series. In: Clinical pathology (Thousand Oaks, Ventura County, Calif.). 2022 ; Vol. 15. pp. 2632010X221139096.

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title = "Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series",

abstract = "BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.",

keywords = "Antibodies, SARS virus, autopsy, case reports, coronavirus, immunohistochemistry",

author = "Lauridsen, \{Karin Holst\} and Olsen, \{Kristine Boisen\} and Lund, \{Eva L{\o}bner\} and Jensen, \{Tomas O\} and Pedersen, \{Thomas Ingemann\} and Harboe, \{Zitta Barrella\} and Valeria Antsupova and Rasmussen, \{Lasse Dam\} and Dennis R{\"o}ser and Jytte Banner and Franck, \{Kristina Tr{\ae}holt\} and \{Vorobieva Solholm Jensen\}, Veronika",

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doi = "10.1177/2632010X221139096",

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journal = "Clinical pathology (Thousand Oaks, Ventura County, Calif.)",

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T1 - Neurological Complications in COVID-19 Patients

T2 - Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series

AU - Lauridsen, Karin Holst

AU - Olsen, Kristine Boisen

AU - Lund, Eva Løbner

AU - Jensen, Tomas O

AU - Pedersen, Thomas Ingemann

AU - Harboe, Zitta Barrella

AU - Antsupova, Valeria

AU - Rasmussen, Lasse Dam

AU - Röser, Dennis

AU - Banner, Jytte

AU - Franck, Kristina Træholt

AU - Vorobieva Solholm Jensen, Veronika

PY - 2022/12

Y1 - 2022/12

N2 - BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.

AB - BACKGROUND: Neurological complications during and after SARS-CoV-2 infection have been frequently described. The detection of either SARS-CoV-2 RNA or specific antibodies against SARS-CoV-2 in cerebrospinal fluid in the context of concomitant neurological manifestations indicates neuroinfection.METHODS AND RESULTS: This is a retrospective descriptive analysis of cerebrospinal fluids and serum samples from 2 hospitalized patients and autopsy findings from 2 patients who died at home. Samples were analysed by 3 independent enzyme-linked immunosorbent assays. Specific antibodies against SARS-CoV-2 were detected in cerebrospinal fluids and paired serum in all 4 cases. Levels of antibodies in cerebrospinal fluids were highest in samples from a deceased man with critical progression of COVID-19 and detectable SARS-CoV-2 viral RNA in cerebrospinal fluid, serum, 4 brain biopsies and 15 additional tissue samples, though immunohistochemical staining for SARS-CoV-2 in brain tissue did not detect the virus.CONCLUSION: Detection of SARS-CoV-2 antibodies in paired serum and cerebrospinal fluid may support the presence of SARS-CoV-2 neuroinflammatory disease in patients with COVID-19 and neurological manifestations.

KW - Antibodies

KW - SARS virus

KW - autopsy

KW - case reports

KW - coronavirus

KW - immunohistochemistry

UR - https://www.scopus.com/pages/publications/85142762469

U2 - 10.1177/2632010X221139096

DO - 10.1177/2632010X221139096

M3 - Journal article

C2 - 36448025

SN - 2632-010X

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SP - 2632010X221139096

JO - Clinical pathology (Thousand Oaks, Ventura County, Calif.)

JF - Clinical pathology (Thousand Oaks, Ventura County, Calif.)

ER -

Neurological Complications in COVID-19 Patients: Can Analysis of Specific Antibodies and Viral RNA in Paired Cerebrospinal Fluid and Serum be Used for Accurate Diagnosis of SARS-CoV-2 Neuroinflammatory Disease? A Case Series

Abstract

Keywords

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