Negative cooperativity between juxtaposed E-box and cAMP/TPA responsive elements in the cholecystokinin gene promoter

I J Rourke; T V Hansen; C Nerlov; J F Rehfeld; F C Nielsen

doi:10.1016/s0014-5793(99)00320-8

Negative cooperativity between juxtaposed E-box and cAMP/TPA responsive elements in the cholecystokinin gene promoter

I J Rourke, T V Hansen, C Nerlov, J F Rehfeld, F C Nielsen

17 Citations (Scopus)

Abstract

The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.

Original language	English
Journal	FEBS Letters
Volume	448
Issue number	1
Pages (from-to)	15-8
Number of pages	4
ISSN	0014-5793
DOIs	https://doi.org/10.1016/s0014-5793(99)00320-8
Publication status	Published - 1 Apr 1999
Externally published	Yes

Keywords

Animals
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Basic-Leucine Zipper Transcription Factors
Cholecystokinin/genetics
Cyclic AMP/metabolism
Cyclic AMP Response Element-Binding Protein/genetics
DNA-Binding Proteins/genetics
Gene Expression Regulation
Helix-Loop-Helix Motifs
Humans
Leucine Zippers
Mice
Molecular Sequence Data
Mutagenesis
Promoter Regions, Genetic
Proto-Oncogene Proteins c-fos/genetics
Proto-Oncogene Proteins c-jun/genetics
Proto-Oncogene Proteins c-myc/genetics
Response Elements
Tetradecanoylphorbol Acetate/metabolism
Transcription Factors/genetics
Tumor Cells, Cultured

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10.1016/s0014-5793(99)00320-8

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title = "Negative cooperativity between juxtaposed E-box and cAMP/TPA responsive elements in the cholecystokinin gene promoter",

abstract = "The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.",

keywords = "Animals, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Basic-Leucine Zipper Transcription Factors, Cholecystokinin/genetics, Cyclic AMP/metabolism, Cyclic AMP Response Element-Binding Protein/genetics, DNA-Binding Proteins/genetics, Gene Expression Regulation, Helix-Loop-Helix Motifs, Humans, Leucine Zippers, Mice, Molecular Sequence Data, Mutagenesis, Promoter Regions, Genetic, Proto-Oncogene Proteins c-fos/genetics, Proto-Oncogene Proteins c-jun/genetics, Proto-Oncogene Proteins c-myc/genetics, Response Elements, Tetradecanoylphorbol Acetate/metabolism, Transcription Factors/genetics, Tumor Cells, Cultured",

author = "Rourke, \{I J\} and Hansen, \{T V\} and C Nerlov and Rehfeld, \{J F\} and Nielsen, \{F C\}",

year = "1999",

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doi = "10.1016/s0014-5793(99)00320-8",

language = "English",

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T1 - Negative cooperativity between juxtaposed E-box and cAMP/TPA responsive elements in the cholecystokinin gene promoter

AU - Rourke, I J

AU - Hansen, T V

AU - Nerlov, C

AU - Rehfeld, J F

AU - Nielsen, F C

PY - 1999/4/1

Y1 - 1999/4/1

N2 - The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.

AB - The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/TRE. Mutation of the E-box enhanced the activation by c-Jun/c-Fos, as well as stimulation by forskolin and bFGF, that acts through the CRE/TRE site. Moreover, c-Jun/c-Fos stimulation was inhibited by co-expression of c-Myc and Max. The results indicate that factors associating with the E-box exhibit a negative cooperative effect on the activation via the CRE/TRE element. We propose that this mechanism plays a significant role in CCK gene transcription and other genes with juxtaposed E-box and CRE/TRE.

KW - Animals

KW - Base Sequence

KW - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors

KW - Basic-Leucine Zipper Transcription Factors

KW - Cholecystokinin/genetics

KW - Cyclic AMP/metabolism

KW - Cyclic AMP Response Element-Binding Protein/genetics

KW - DNA-Binding Proteins/genetics

KW - Gene Expression Regulation

KW - Helix-Loop-Helix Motifs

KW - Humans

KW - Leucine Zippers

KW - Mice

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Promoter Regions, Genetic

KW - Proto-Oncogene Proteins c-fos/genetics

KW - Proto-Oncogene Proteins c-jun/genetics

KW - Proto-Oncogene Proteins c-myc/genetics

KW - Response Elements

KW - Tetradecanoylphorbol Acetate/metabolism

KW - Transcription Factors/genetics

KW - Tumor Cells, Cultured

UR - https://www.scopus.com/pages/publications/0033061295

U2 - 10.1016/s0014-5793(99)00320-8

DO - 10.1016/s0014-5793(99)00320-8

M3 - Journal article

C2 - 10217400

SN - 0014-5793

VL - 448

SP - 15

EP - 18

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Negative cooperativity between juxtaposed E-box and cAMP/TPA responsive elements in the cholecystokinin gene promoter

Abstract

Keywords

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