Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Multiscale modeling of innate immune receptors: Endotoxin recognition and regulation by host defense peptides

Research output: Contribution to journalReviewpeer-review

Harvard

APA

CBE

MLA

Vancouver

Author

Holdbrook, Daniel A ; Huber, Roland G ; Marzinek, Jan K ; Stubbusch, Astrid ; Schmidtchen, Artur ; Bond, Peter J. / Multiscale modeling of innate immune receptors : Endotoxin recognition and regulation by host defense peptides. In: Pharmacological Research. 2019 ; Vol. 147. pp. 104372.

Bibtex

@article{b2ea20b8459e4ef9a3f53ea7df1aa753,
title = "Multiscale modeling of innate immune receptors: Endotoxin recognition and regulation by host defense peptides",
abstract = "The innate immune system provides a first line of defense against foreign microorganisms, and is typified by the Toll-like receptor (TLR) family. TLR4 is of particular interest, since over-stimulation of its pathway by excess lipopolysaccharide (LPS) molecules from the outer membranes of Gram-negative bacteria can result in sepsis, which causes millions of deaths each year. In this review, we outline our use of molecular simulation approaches to gain a better understanding of the determinants of LPS recognition, towards the search for novel immunotherapeutics. We first describe how atomic-resolution simulations have enabled us to elucidate the regulatory conformational changes in TLR4 associated with different LPS analogues, and hence a means to rationalize experimental structure-activity data. Furthermore, multiscale modelling strategies have provided a detailed description of the thermodynamics and intermediate structures associated with the entire TLR4 relay - which consists of a number of transient receptor/coreceptor complexes - allowing us trace the pathway of LPS transfer from bacterial membranes to the terminal receptor complex at the plasma membrane surface. Finally, we describe our efforts to leverage these computational models, in order to elucidate previously undisclosed anti-inflammatory mechanisms of endogenous host-defense peptides found in wounds. Collectively, this work represents a promising avenue for the development of novel anti-septic treatments, inspired by nature's innate defense strategies.",
author = "Holdbrook, {Daniel A} and Huber, {Roland G} and Marzinek, {Jan K} and Astrid Stubbusch and Artur Schmidtchen and Bond, {Peter J}",
note = "Copyright {\textcopyright} 2019 Elsevier Ltd. All rights reserved.",
year = "2019",
month = sep,
doi = "10.1016/j.phrs.2019.104372",
language = "English",
volume = "147",
pages = "104372",
journal = "Pharmacological Research",
issn = "1043-6618",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Multiscale modeling of innate immune receptors

T2 - Endotoxin recognition and regulation by host defense peptides

AU - Holdbrook, Daniel A

AU - Huber, Roland G

AU - Marzinek, Jan K

AU - Stubbusch, Astrid

AU - Schmidtchen, Artur

AU - Bond, Peter J

N1 - Copyright © 2019 Elsevier Ltd. All rights reserved.

PY - 2019/9

Y1 - 2019/9

N2 - The innate immune system provides a first line of defense against foreign microorganisms, and is typified by the Toll-like receptor (TLR) family. TLR4 is of particular interest, since over-stimulation of its pathway by excess lipopolysaccharide (LPS) molecules from the outer membranes of Gram-negative bacteria can result in sepsis, which causes millions of deaths each year. In this review, we outline our use of molecular simulation approaches to gain a better understanding of the determinants of LPS recognition, towards the search for novel immunotherapeutics. We first describe how atomic-resolution simulations have enabled us to elucidate the regulatory conformational changes in TLR4 associated with different LPS analogues, and hence a means to rationalize experimental structure-activity data. Furthermore, multiscale modelling strategies have provided a detailed description of the thermodynamics and intermediate structures associated with the entire TLR4 relay - which consists of a number of transient receptor/coreceptor complexes - allowing us trace the pathway of LPS transfer from bacterial membranes to the terminal receptor complex at the plasma membrane surface. Finally, we describe our efforts to leverage these computational models, in order to elucidate previously undisclosed anti-inflammatory mechanisms of endogenous host-defense peptides found in wounds. Collectively, this work represents a promising avenue for the development of novel anti-septic treatments, inspired by nature's innate defense strategies.

AB - The innate immune system provides a first line of defense against foreign microorganisms, and is typified by the Toll-like receptor (TLR) family. TLR4 is of particular interest, since over-stimulation of its pathway by excess lipopolysaccharide (LPS) molecules from the outer membranes of Gram-negative bacteria can result in sepsis, which causes millions of deaths each year. In this review, we outline our use of molecular simulation approaches to gain a better understanding of the determinants of LPS recognition, towards the search for novel immunotherapeutics. We first describe how atomic-resolution simulations have enabled us to elucidate the regulatory conformational changes in TLR4 associated with different LPS analogues, and hence a means to rationalize experimental structure-activity data. Furthermore, multiscale modelling strategies have provided a detailed description of the thermodynamics and intermediate structures associated with the entire TLR4 relay - which consists of a number of transient receptor/coreceptor complexes - allowing us trace the pathway of LPS transfer from bacterial membranes to the terminal receptor complex at the plasma membrane surface. Finally, we describe our efforts to leverage these computational models, in order to elucidate previously undisclosed anti-inflammatory mechanisms of endogenous host-defense peptides found in wounds. Collectively, this work represents a promising avenue for the development of novel anti-septic treatments, inspired by nature's innate defense strategies.

U2 - 10.1016/j.phrs.2019.104372

DO - 10.1016/j.phrs.2019.104372

M3 - Review

C2 - 31351116

VL - 147

SP - 104372

JO - Pharmacological Research

JF - Pharmacological Research

SN - 1043-6618

ER -

ID: 59238232