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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients

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  4. Diagnosis of biofilm infections in cystic fibrosis patients

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  2. Methods for collecting upper respiratory tract specimens for COVID-19 diagnostics

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  3. COVID-19 infection rate among tertiary referral center otorhinolaryngology healthcare workers

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Phenotypic and genotypic diversifications of Pseudomonas aeruginosa in the airways of patients with cystic fibrosis (CF) promote long-term survival of bacteria during chronic lung infection. Twelve clonally related, sequential mucoid and non-mucoid paired P. aeruginosa isolates obtained from three Danish CF patients were investigated. The in vitro biofilm formation capacity was studied under static and flow through conditions and the global gene expression profiles were investigated by Affymetrix GeneChip. Regulatory genes of alginate production and quorum sensing (QS) system were sequenced and measurements of the alginate production and the detection of the QS signal molecules were performed. Comparisons of mucoid and non-mucoid isolates from early and late stages of the infection showed that the mucoid phenotype maintained over a decade the capacity to form in vitro biofilm and showed an unaltered transcriptional profile, whereas substantial alterations in the transcriptional profiles and loss of the capacity to form in vitro biofilms were observed in corresponding isolates of the non-mucoid phenotype. The conserved gene expression pattern in the mucoid isolates vs the diversity of changes in non-mucoid isolates observed in this particular P. aeruginosa clone reflects different adaptation strategies used by these two phenotypes in the different niches of the CF lung environment.
Original languageEnglish
JournalActa Pathologica Microbiologica et Immunologica Scandinavica
Volume119
Issue number4-5
Pages (from-to)263-74
Number of pages12
ISSN0903-4641
DOIs
Publication statusPublished - 1 Apr 2011

    Research areas

  • Alginates, Bacterial Proteins, Biofilms, Chronic Disease, Cystic Fibrosis, Denmark, Gene Expression Profiling, Genes, Bacterial, Genotype, Glucuronic Acid, Hexuronic Acids, Humans, Lung Diseases, Mutation, Oligonucleotide Array Sequence Analysis, Phenotype, Pseudomonas Infections, Pseudomonas aeruginosa, Quorum Sensing, Sigma Factor, Trans-Activators

ID: 32661216