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Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

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Harvard

Silva, S, Altmannova, V, Luke-Glaser, S, Henriksen, P, Gallina, I, Yang, X, Choudhary, C, Luke, B, Krejci, L & Lisby, M 2016, 'Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance', Genes & Development, vol. 30, no. 6, pp. 700-17. https://doi.org/10.1101/gad.276204.115

APA

Silva, S., Altmannova, V., Luke-Glaser, S., Henriksen, P., Gallina, I., Yang, X., Choudhary, C., Luke, B., Krejci, L., & Lisby, M. (2016). Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance. Genes & Development, 30(6), 700-17. https://doi.org/10.1101/gad.276204.115

CBE

Silva S, Altmannova V, Luke-Glaser S, Henriksen P, Gallina I, Yang X, Choudhary C, Luke B, Krejci L, Lisby M. 2016. Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance. Genes & Development. 30(6):700-17. https://doi.org/10.1101/gad.276204.115

MLA

Vancouver

Author

Silva, Sonia ; Altmannova, Veronika ; Luke-Glaser, Sarah ; Henriksen, Peter ; Gallina, Irene ; Yang, Xuejiao ; Choudhary, Chunaram ; Luke, Brian ; Krejci, Lumir ; Lisby, Michael. / Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance. In: Genes & Development. 2016 ; Vol. 30, No. 6. pp. 700-17.

Bibtex

@article{7131eeb9b0a740e8b9de49ec43c3d25d,
title = "Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance",
abstract = "Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance. ",
keywords = "Crossing Over, Genetic/genetics, DEAD-box RNA Helicases/genetics, Gene Deletion, Protein Transport, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics, Stress, Physiological/genetics, Telomere Homeostasis/genetics, Telomere-Binding Proteins/genetics",
author = "Sonia Silva and Veronika Altmannova and Sarah Luke-Glaser and Peter Henriksen and Irene Gallina and Xuejiao Yang and Chunaram Choudhary and Brian Luke and Lumir Krejci and Michael Lisby",
note = "{\textcopyright} 2016 Silva et al.; Published by Cold Spring Harbor Laboratory Press.",
year = "2016",
month = mar,
day = "15",
doi = "10.1101/gad.276204.115",
language = "English",
volume = "30",
pages = "700--17",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press Publications Department",
number = "6",

}

RIS

TY - JOUR

T1 - Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance

AU - Silva, Sonia

AU - Altmannova, Veronika

AU - Luke-Glaser, Sarah

AU - Henriksen, Peter

AU - Gallina, Irene

AU - Yang, Xuejiao

AU - Choudhary, Chunaram

AU - Luke, Brian

AU - Krejci, Lumir

AU - Lisby, Michael

N1 - © 2016 Silva et al.; Published by Cold Spring Harbor Laboratory Press.

PY - 2016/3/15

Y1 - 2016/3/15

N2 - Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.

AB - Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans. Here, we identify Mte1 as a novel interactor of the Mph1 helicase in Saccharomyces cerevisiae. In vitro, Mte1 (Mph1-associated telomere maintenance protein 1) binds directly to DNA with a preference for branched molecules such as D loops and fork structures. In addition, Mte1 stimulates the helicase and fork regression activities of Mph1 while inhibiting the ability of Mph1 to dissociate recombination intermediates. Deletion of MTE1 reduces crossover recombination and suppresses the sensitivity of mph1Δ mutant cells to replication stress. Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres. Taken together, our data indicate that Mte1 plays a role in regulation of crossover recombination, response to replication stress, and telomere maintenance.

KW - Crossing Over, Genetic/genetics

KW - DEAD-box RNA Helicases/genetics

KW - Gene Deletion

KW - Protein Transport

KW - Saccharomyces cerevisiae/genetics

KW - Saccharomyces cerevisiae Proteins/genetics

KW - Stress, Physiological/genetics

KW - Telomere Homeostasis/genetics

KW - Telomere-Binding Proteins/genetics

U2 - 10.1101/gad.276204.115

DO - 10.1101/gad.276204.115

M3 - Journal article

C2 - 26966248

VL - 30

SP - 700

EP - 717

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 6

ER -

ID: 54999837